Poly(2-dimethylamino ethylmethacrylate)-based polymers to camouflage red blood cell antigens
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Poly(2-dimethylamino-ethylmethacrylate) (PDMAEMA) is a cationic polymer when dissolved in a 7.4 pH fluid. Owing to its ionic nature, this polycation interacts with the negatively charged cell membrane surface of red blood cells (RBCs). The electrostatic self-assembly of PDMAEMA on RBCs membrane can be employed for inducing the formation of a polymeric shield camouflaging blood group antigens on RBCs as a valuable strategy for developing universal RBCs for blood transfusion. The purpose of this research was to evaluate the camouflaging ability of PDMAEMA homopolymers and PDMAEMA-copoly( ethylene glycol) copolymers differing in molecular weight and architecture. Surprisingly, the PDMAEMAs caused a partially masking, no masking, and sensitization of the same RBCs population. The MW and architecture of the polymers as well as temperature of PDMAEMA-RBCs treatment influenced the results observed. Herein, the very particular reactivity of PDMAEMAs and RBCs is analyzed and discussed. © 2012 American Chemical Society.
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Blood group antigen; Blood transfusion; Cationic polymers; Cell membrane surface; Electrostatic self assembly; Ethylmethacrylate; Ionic nature; PDMAEMA; Polycations; Red blood cell; Antigens; Blood; Cells; Ethylene glycol; Polymers; erythrocyte antigen; macrogol; poly[2 (dimethylamino)ethyl methacrylate]; polycation; article; cell membrane; cell surface; controlled study; erythrocyte; material coating; molecular weight; particle size; pH; priority journal; static electricity; surface charge; Blood Group Antigens; Coated Materials, Biocompatible; Erythrocytes; Fluorescence; Humans; Methacrylates; Nylons; Surface Properties
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