Transgenic carrot tap roots expressing an immunogenic F1-V fusion protein from Yersinia pestis are immunogenic in mice
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Expression of the protective F1 and V antigens of Yersinia pestis, as a fusion protein, in carrot was pursued in an effort to develop an alternative vaccine production system against the serious plague disease. Transgenic carrot plants carrying the F1-V encoding gene were developed via Agrobacterium-mediated transformation. Presence, integration, and expression of the F1-V encoding gene were confirmed by polymerase chain reaction (PCR), DNA gel blot analysis, and reverse-transcriptase (RT)-PCR analyses, respectively. An ELISA assay confirmed the antigenicity of the plant-derived F1-V fusion protein. Immunogenicity was evaluated subcutaneously in mice using a soluble protein extract of freeze-dried transgenic carrot. Significant antibody levels were detected following immunization. These results demonstrated that the F1-V protein could be expressed in carrot tap roots, and that the carrot F1-V recombinant protein retained its antigenicity and immunogenicity. © 2010 Elsevier GmbH.
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Antigenic protein; Plague; Plant based-vaccine; Transgenic carrot bacterial antigen; bacterial protein; caf1 protein, Yersinia pestis; hybrid protein; LcrV protein, Yersinia; plague vaccine; pore forming cytotoxic protein; animal; article; Bagg albino mouse; carrot; enzyme linked immunosorbent assay; genetics; immunology; metabolism; mouse; plague; plant root; reverse transcription polymerase chain reaction; transgenic plant; Yersinia pestis; Animals; Antigens, Bacterial; Bacterial Proteins; Daucus carota; Enzyme-Linked Immunosorbent Assay; Mice; Mice, Inbred BALB C; Plague; Plague Vaccine; Plant Roots; Plants, Genetically Modified; Pore Forming Cytotoxic Proteins; Recombinant Fusion Proteins; Reverse Transcriptase Polymerase Chain Reaction; Yersinia pestis; Agrobacterium; Daucus carota; Mus; Rhizobium; Yersinia pestis
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