Analysis of expression and function of the inhibitory receptor ILT2 in lymphocytes from patients with autoimmune thyroid disease
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Objective: Autoimmune thyroid disease (AITD) is characterized by different defects in immunoregulatory mechanisms. The immunoglobulin-like transcript receptor 2 (ILT2) or leukocyte Ig-like receptor 1 (LIRB1/CD85j) exerts an important immunoregulatory role. We hypothesized that the lymphocytes from AITD patients have a diminished expression and function of ILT2. The aim of this study was to investigate the expression and function of ILT2 in lymphocytes from patients with AITD. Design and methods: In this study, 18 patients with Hashimoto%27s thyroiditis (HT), 20 with Graves%27 disease, and 26 healthy controls were studied. ILT2 expression was analyzed by flow cytometry and immunohistochemistry in peripheral blood mononuclear cells (PBMC) and thyroid tissue. The regulatory function of ILT2 was assessed by an assay of inhibition of lymphocyte proliferation and by an analysis of cell cycle progression. The effect of ILT2 on cytokine synthesis was also evaluated. Results: We found a significant increased expression of ILT2 by lymphocytes in AITD patients. ILT2 was also detected in the leukocyte infiltrate of thyroid tissue from HT patients. On the contrary, a significant diminished inhibitory activity of ILT2 on cell proliferation was observed in AITD patients. In addition, PBMC from AITD patients showed a diminished synthesis of interleukin 10 on ILT2 engagement. Conclusions: The abnormal expression and function of ILT2 detected in AITD suggests that this receptor may participate in the pathogenesis of this condition. © 2011 European Society of Endocrinology.
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Objective: Autoimmune thyroid disease (AITD) is characterized by different defects in immunoregulatory mechanisms. The immunoglobulin-like transcript receptor 2 (ILT2) or leukocyte Ig-like receptor 1 (LIRB1/CD85j) exerts an important immunoregulatory role. We hypothesized that the lymphocytes from AITD patients have a diminished expression and function of ILT2. The aim of this study was to investigate the expression and function of ILT2 in lymphocytes from patients with AITD. Design and methods: In this study, 18 patients with Hashimoto's thyroiditis (HT), 20 with Graves' disease, and 26 healthy controls were studied. ILT2 expression was analyzed by flow cytometry and immunohistochemistry in peripheral blood mononuclear cells (PBMC) and thyroid tissue. The regulatory function of ILT2 was assessed by an assay of inhibition of lymphocyte proliferation and by an analysis of cell cycle progression. The effect of ILT2 on cytokine synthesis was also evaluated. Results: We found a significant increased expression of ILT2 by lymphocytes in AITD patients. ILT2 was also detected in the leukocyte infiltrate of thyroid tissue from HT patients. On the contrary, a significant diminished inhibitory activity of ILT2 on cell proliferation was observed in AITD patients. In addition, PBMC from AITD patients showed a diminished synthesis of interleukin 10 on ILT2 engagement. Conclusions: The abnormal expression and function of ILT2 detected in AITD suggests that this receptor may participate in the pathogenesis of this condition. © 2011 European Society of Endocrinology.
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immunoglobulin like transcript receptor 2; immunoglobulin receptor; interleukin 10; interleukin 2; unclassified drug; lymphocyte receptor; immunoglobulin receptor; interleukin 10; leukocyte antigen; LILRB1 protein, human; article; B lymphocyte; CD4 T lymphocyte; CD8 T lymphocyte; cell cycle progression; cell infiltration; clinical article; controlled study; cytokine production; flow cytometry; Graves disease; Hashimoto disease; human; human cell; human tissue; immunohistochemistry; immunopathogenesis; leukocyte; lymphocyte proliferation; peripheral blood mononuclear cell; priority journal; protein expression; protein function; regulatory mechanism; thyroid gland; autoimmune thyroiditis; biosynthesis; cell proliferation; drug effect; Graves disease; Hashimoto disease; immunology; lymphocyte; metabolism; mononuclear cell; pathology; physiology; Antigens, CD; Cell Proliferation; Graves Disease; Hashimoto Disease; Humans; Interleukin-10; Leukocytes, Mononuclear; Lymphocytes; Receptors, Immunologic; Thyroid Gland
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immunoglobulin like transcript receptor 2; immunoglobulin receptor; interleukin 10; interleukin 2; unclassified drug; lymphocyte receptor; immunoglobulin receptor; interleukin 10; leukocyte antigen; LILRB1 protein, human; article; B lymphocyte; CD4+ T lymphocyte; CD8+ T lymphocyte; cell cycle progression; cell infiltration; clinical article; controlled study; cytokine production; flow cytometry; Graves disease; Hashimoto disease; human; human cell; human tissue; immunohistochemistry; immunopathogenesis; leukocyte; lymphocyte proliferation; peripheral blood mononuclear cell; priority journal; protein expression; protein function; regulatory mechanism; thyroid gland; autoimmune thyroiditis; biosynthesis; cell proliferation; drug effect; Graves disease; Hashimoto disease; immunology; lymphocyte; metabolism; mononuclear cell; pathology; physiology; Antigens, CD; Cell Proliferation; Graves Disease; Hashimoto Disease; Humans; Interleukin-10; Leukocytes, Mononuclear; Lymphocytes; Receptors, Immunologic; Thyroid Gland
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