Influence of human cytomegalovirus infection on the NK cell receptor repertoire in children
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Human cytomegalovirus (hCMV) infection is usually asymptomatic but may cause disease in immunocompromised hosts. It has been reported that hCMV infection may shape the NK cell receptor (NKR) repertoire in adult individuals, promoting a variable expansion of the CD94/NKG2C NK cell subset.We explored the possible relationship between this viral infection and the expression pattern of different NKR including CD94/NKG2C, CD94/NKG2A, immunoglobulin-like transcript 2 (ILT2, CD85j), KIR2DL1/2DS1, KIR3DL1, and CD161 in peripheral blood lymphocytes from healthy children, seropositive (n = 21) and seronegative (n = 20) for hCMV. Consistent with previous observations in adults, a positive serology for hCMV was associated with increased numbers of NKG2C NK and T cells as well as with ILT21 T lymphocytes. Moreover, the proportions of CD1611 and NKG2C CD56-CD3- NK cells also tended to be increased in hCMV individuals. Excretion of the virus was associated with higher proportions of NKG2C NK cells. Altogether, these data reveal that hCMV may have a profound influence on the NKR repertoire in early childhood. © 2010 Wiley-VCH Verlag GmbH & Co. KGaA.
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Human cytomegalovirus (hCMV) infection is usually asymptomatic but may cause disease in immunocompromised hosts. It has been reported that hCMV infection may shape the NK cell receptor (NKR) repertoire in adult individuals, promoting a variable expansion of the CD94/NKG2C%2b NK cell subset.We explored the possible relationship between this viral infection and the expression pattern of different NKR including CD94/NKG2C, CD94/NKG2A, immunoglobulin-like transcript 2 (ILT2, CD85j), KIR2DL1/2DS1, KIR3DL1, and CD161 in peripheral blood lymphocytes from healthy children, seropositive (n = 21) and seronegative (n = 20) for hCMV. Consistent with previous observations in adults, a positive serology for hCMV was associated with increased numbers of NKG2C%2b NK and T cells as well as with ILT21 T lymphocytes. Moreover, the proportions of CD1611 and NKG2C%2bCD56-CD3- NK cells also tended to be increased in hCMV%2b individuals. Excretion of the virus was associated with higher proportions of NKG2C%2b NK cells. Altogether, these data reveal that hCMV may have a profound influence on the NKR repertoire in early childhood. © 2010 Wiley-VCH Verlag GmbH %26amp; Co. KGaA.
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C-type lectins; Human cytomegalovirus; Killer immunoglobulin-like receptor (KIR); NK cell; T cell CD161 antigen; CD3 antigen; CD56 antigen; CD94 antigen; killer cell immunoglobulin like receptor 2DL1; killer cell immunoglobulin like receptor 3DL1; natural killer cell receptor; natural killer cell receptor NKG2A; natural killer cell receptor NKG2C; unclassified drug; antigen expression; article; child; cytomegalovirus infection; female; human; infant; male; natural killer T cell; nucleotide sequence; preschool child; priority journal; school child; serology; T lymphocyte; Antibodies, Viral; Antigens, CD; Child; Child, Preschool; Cytomegalovirus; Cytomegalovirus Infections; Female; Gene Expression Regulation, Viral; Humans; Immunoglobulin G; Infant; Killer Cells, Natural; Male; NK Cell Lectin-Like Receptor Subfamily B; NK Cell Lectin-Like Receptor Subfamily C; NK Cell Lectin-Like Receptor Subfamily D; Receptors, Immunologic; Receptors, Natural Killer Cell; Saliva; Urine
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