abstract

• Infiltration of biomacromolecules into porous silicon photonic architectures results in biofunctionalized structures with unique properties. Characterization of their optical response and performance optimization in biomacromolecular detection and biophotonic application require a combination of optical and structural studies. Nonlinear optical microscopy is applied to study porous silicon microcavities with and without infiltrated glucose oxidase. The infiltrated protein acts as an internal two-photon-excited fluorescence emitter and second harmonic generator, enabling the in-depth visualization of the porous structure. Enhanced second harmonic generation and fluorescence emission by the porous silicon structure is experimentally associated with the defect layer. © 2009 American Institute of Physics.

authors

• Agarwal V.
• Cloitre T.
• Gergely C.
• Martin M.
• Palestino Escobedo Alma Gabriela
• Zimányi L.

publication date

• 2009-01-01

funding provided via

• Hungarian Scientific Research Fund, OTKA  Grant

published in

• Applied Physics Letters  Journal

keywords

• Biomacromolecules; Biophotonic applications; Defect layers; Fluorescence emission; Nonlinear optical microscopy; Nonlinear photoemission; Optical response; Performance optimizations; Porous silicon microcavities; Porous silicon structures; Porous structures; Second harmonic generation; Second harmonics; Silicon photonics; Spatial resolution; Structural studies; Two-photon excited fluorescence; Fluorescence; Glucose; Glucose oxidase; Glucose sensors; Harmonic generation; Medical imaging; Microcavities; Nonlinear optics; Optical microscopy; Porous silicon

Digital Object Identifier (DOI)

• 10.1063/1.3148698

start page

end page

volume

• 94

issue

• 22