The effect of Emdogain® and 24%25 EDTA root conditioning on periodontal healing of replanted dog's teeth
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Controversies still exist as for the regenerative role of enamel matrix derivatives and the need for removal of the periodontal ligament in replanted teeth. The purpose of this study was to evaluate the effect of Emdogain® and 24%25 ethylenediamine tetraacetic acid (EDTA) root conditioning on periodontal healing of replanted dog%27s teeth. Teeth were extracted, endodontically treated and preconditioned as follows: group 1, Emdogain®; group 2, Emdogain® EDTA and group 3, EDTA. Teeth were replanted after 30 min extraoral time, splinted for 15 days and animals sacrificed after 8 weeks of observation. Histological evaluation was performed using hematoxylin/eosin and Masson trichrome and results scored based on previously reported criteria for histological evaluation. Replacement root resorption was histologically diagnosed in all groups except in the negative control. A parametric analysis showed no statistically significant differences between experimental groups. Root preconditioning with Emdogain® alone or in combination with 24%25 EDTA showed no evidence of regeneration of collagen fibers and consequently did not prevent the development of replacement root resorption on replanted dog%27s teeth. © 2009 John Wiley & Sons A/S.
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Controversies still exist as for the regenerative role of enamel matrix derivatives and the need for removal of the periodontal ligament in replanted teeth. The purpose of this study was to evaluate the effect of Emdogain® and 24%25 ethylenediamine tetraacetic acid (EDTA) root conditioning on periodontal healing of replanted dog%27s teeth. Teeth were extracted, endodontically treated and preconditioned as follows: group 1, Emdogain®; group 2, Emdogain® %2b EDTA and group 3, EDTA. Teeth were replanted after 30 min extraoral time, splinted for 15 days and animals sacrificed after 8 weeks of observation. Histological evaluation was performed using hematoxylin/eosin and Masson trichrome and results scored based on previously reported criteria for histological evaluation. Replacement root resorption was histologically diagnosed in all groups except in the negative control. A parametric analysis showed no statistically significant differences between experimental groups. Root preconditioning with Emdogain® alone or in combination with 24%25 EDTA showed no evidence of regeneration of collagen fibers and consequently did not prevent the development of replacement root resorption on replanted dog%27s teeth. © 2009 John Wiley %26 Sons A/S.
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Controversies still exist as for the regenerative role of enamel matrix derivatives and the need for removal of the periodontal ligament in replanted teeth. The purpose of this study was to evaluate the effect of Emdogain® and 24%25 ethylenediamine tetraacetic acid (EDTA) root conditioning on periodontal healing of replanted dog's teeth. Teeth were extracted, endodontically treated and preconditioned as follows: group 1, Emdogain®; group 2, Emdogain® %2b EDTA and group 3, EDTA. Teeth were replanted after 30 min extraoral time, splinted for 15 days and animals sacrificed after 8 weeks of observation. Histological evaluation was performed using hematoxylin/eosin and Masson trichrome and results scored based on previously reported criteria for histological evaluation. Replacement root resorption was histologically diagnosed in all groups except in the negative control. A parametric analysis showed no statistically significant differences between experimental groups. Root preconditioning with Emdogain® alone or in combination with 24%25 EDTA showed no evidence of regeneration of collagen fibers and consequently did not prevent the development of replacement root resorption on replanted dog's teeth. © 2009 John Wiley %26 Sons A/S.
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chelating agent; edetic acid; enamel matrix proteins; enamel protein; animal; article; dog; drug effect; endodontics; evaluation; periodontal ligament; randomization; regeneration; tooth disease; tooth injury; tooth root; Animals; Chelating Agents; Dental Enamel Proteins; Dogs; Edetic Acid; Periodontal Ligament; Random Allocation; Regeneration; Root Resorption; Tooth Avulsion; Tooth Replantation; Tooth Root
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