Polyamine metabolism in maize tumors induced by Ustilago maydis
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Alterations occurring in polyamine metabolism of maize in tumors formed during the interaction with the biotrophic pathogenic fungus Ustilago maydis were analyzed. During the process, a striking increase in maize polyamine biosynthesis, mainly free and conjugated putrescine occurred in the tumors induced by the fungus, and in the neighbor plant tissues. This increase correlated with an activation mainly of Adc, Samdc1, Zmsamdc2 and Zmsamdc3, but not of Zmodc, Zmspds1 and Zmspds2 genes, and an elevation in arginine decarboxylase activity, confirming a predominant role of this enzyme in the process. Evidences for a possible contribution of spermidine and spermine degradation by polyamine oxidase activity, probably related to cell wall stiffening or lignification during tumor growth, were also obtained. It is suggested that polyamines, mainly putrescine, might play an active role in the pathosystem maize-U. maydis. © 2008 Elsevier Masson SAS. All rights reserved.
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Biotic stress; Free and conjugated polyamines; Plant tumors; Ustilago maydis; Zea mays arginine decarboxylase; carboxylyase; ornithine decarboxylase; oxidoreductase; plant RNA; polyamine oxidase; putrescine; article; enzymology; gene; gene expression profiling; gene expression regulation; genetics; growth, development and aging; maize; metabolism; microbiology; plant tumor; reverse transcription polymerase chain reaction; Ustilago; Carboxy-Lyases; Gene Expression Profiling; Gene Expression Regulation, Plant; Genes, Plant; Ornithine Decarboxylase; Oxidoreductases Acting on CH-NH Group Donors; Plant Tumors; Putrescine; Reverse Transcriptase Polymerase Chain Reaction; RNA, Plant; Ustilago; Zea mays; Fungi; Ustilago maydis; Zea mays
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