Optimization of human interferon gamma production in Escherichia coli by response surface methodology

The production of human interferon gamma (hIFN-γ) using a synthetic gene in Escherichia coli BL21-SI was optimized by response surface methodology (RSM) and a Box-Behnken design. The process variables studied were temperature, biomass concentration at induction time and the NaCl concentration as inducer. According to the Box-Behnken design, a second order response function was developed. The optimal expression conditions were a temperature of 32.6°C, induction biomass of 0.31 g/L and 0.3 M NaCl in minimal medium. The model prediction for the maximum hIFN-γ production was 77.3 mg/L, which corresponded satisfactorily with the experimental data. The hIFN-γ concentration attained under optimized conditions was 13-times higher than that obtained using the non-optimized conditions. We conclude that RSM is an effective method for the optimization of recombinant protein expression using synthetic genes in E. coli. © KSBB.

Escherichia coli BL21-SI; Minimum medium; Synthetic gene; Therapeutic protein recombinant gamma interferon; sodium chloride; synthetic DNA; article; bacterial gene; biomass production; biotechnological procedures; biotechnological production; box behnken design; concentration response; controlled study; cytokine production; Escherichia coli; intermethod comparison; nonhuman; process design; process optimization; protein expression; response surface method; temperature; Escherichia coli

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