Modified penicillin acylase signal peptide allows the periplasmic production of soluble human interferon-γ but not of soluble human interleukin-2 by the Tat pathway in Escherichia coli Article uri icon

abstract

  • Production of periplasmic human interferon-γ (hINF-γ) and human interleukin-2 (hIL-2) by the Tat translocation pathway in Escherichia coli BL21-SI was evaluated. The expression was obtained using the pEMR vector which contains the Tat-dependent modified penicillin acylase signal peptide (mSPpac) driven by the T7 promoter. The mSPpac-hINF-γ was processed and the protein was transported to periplasm. Up to 30.1%25 of hINF-γ was found in the periplasmic soluble fraction, whereas only 15%25 of the mSPpac-hIL-2 was processed, but hIL-2 was not found in the periplasmic soluble fraction. © 2007 Springer Science%2bBusiness Media B.V.

publication date

  • 2007-01-01