Impact of opportunistic Mycobacterium tuberculosis infection on the phenotype of peripheral blood T cells of AIDS patients
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While the detrimental consequences of opportunistic tuberculosis (TB) in the course and outcome of HIV-1 infection are well studied, little information about the impact of the mycobacterial infection on the phenotype of T lymphocytes is available. In this study we analyzed by cytofluorimetry the peripheral blood T cell phenotype of 13 patients with AIDS, 23 HIV-1 negative patients with active pulmonary TB, nine HIV-1/Mycobacterium tuberculosis coinfected individuals, and 21 age- and sex-matched healthy controls. CD4%2b T cells were equally depleted in AIDS and coinfection (P<0.001). The findings suggest a rescuing effect of the added mycobacterial infection. CD3 T cell loss was not observed in coinfection, whereas it was severe in AIDS (P<0.001). Similar (albeit less striking) effects were observed with other markers (CD45RA, CD45RO, and CD27) that were diminished in CD4%2b T cells of AIDS patients. Apparent detrimental effects of the added mycobacterial infection were the increased expression of the proapoptotic molecule CD95 on CD4%2b T cells, and decreased expression of the major costimulatory molecule CD28 on CD8%2b T cells. In this work we show that M. tuberculosis infection modifies the T cell phenotype of the HIV-1 infected individual. © 2006 Wiley-Liss, Inc.
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AIDS; Flow cytometry; HIV-1; T cells; Tuberculosis CD27 antigen; CD28 antigen; CD3 antigen; CD45RA antigen; CD45RO antigen; Fas antigen; acquired immune deficiency syndrome; adult; aged; AIDS patient; antigen expression; apoptosis; article; CD4+ T lymphocyte; CD8+ T lymphocyte; clinical article; comorbidity; controlled study; cytofluorometry; disease course; fatality; female; human; human cell; Human immunodeficiency virus 1; Human immunodeficiency virus infected patient; Human immunodeficiency virus infection; male; Mycobacterium tuberculosis; opportunistic infection; peripheral lymphocyte; phenotype; T lymphocyte; tuberculosis; Adult; Aged; AIDS-Related Opportunistic Infections; CD4-Positive T-Lymphocytes; Female; Flow Cytometry; HIV-1; Humans; Immunocompromised Host; Immunophenotyping; Male; Middle Aged; Tuberculosis, Pulmonary; Human immunodeficiency virus 1; Mycobacterium tuberculosis
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