Pigment production by callus of Mammillaria candida Scheidweiler (cactaceae) [Producción de pigmentos por callos de Mammillaria candida Scheidweiler (cactaceae)]
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abstract
In order to obtain natural colors from cacti without affecting natural populations, cultures of highly pigmented callus of Mammillaria candida were established. The calli were cultured in Murashige and Skoog medium with 3 mg L-1 of 2, 4-dichlorophenoxiacetic acid, naphthalene acetic acid, or chlorophenoxiacetic acid, alone or in combination with 0.1 mg L-1 kinetin, kinetin riboside, or benzyladenine. About 80%25 of the calli were pigmented in media with auxins, and 50%25 in media with auxins and cytokinins (p≤0.05). Tissue pigmentation increased four times in medium with 50 and 100 g L-1 glucose or sucrose (abiotic stress) and with fungal extracts (biotic stress). The spectrophotometric analysis showed that the pigment corresponds a modified betaxanthine.
