Simultaneous production of biohydrogen, bioethanol, and bio-2,3-butanediol by Enterobacter cloacae K1 isolated from koala using glucose as substrate
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abstract
Enterobacter cloacae K1 isolated from koala (Phascolarctos cinereus) was used for the simultaneous production of glucose-based biofuels. Genomic analysis showed genes related to bio-2,3-butanediol (BDO), bioethanol (BE), and biohydrogen (BH) production, such as hydrogenase 3, α-acetolactate decarboxylase, α-acetolactate synthase, and three alcohol dehydrogenases. To optimise the multi-biofuel production, a central composite design was applied to assess the effect of glucose concentration, pH, and temperature. Optimal conditions were an initial pH of 9.2, 28.5 °C, and 78.9 g dm−3 glucose, obtaining 22.79 ± 0.32 g dm−3 BDO, 10.8 ± 0.22 g dm−3 BE, and 6378.4 ± 857.8 cm3 dm−3 of BH. Maximum yields of 0.31 g g−1, 0.16 g g−1, and 0.49 mol mol−1 were achieved for BDO, BE, and BH, respectively. The main factor that affected the response variables was the square term of temperature. This work strengthens the novel approach of the simultaneous production of biofuels by using the metabolic abilities of E. cloacae K1.
