Cytosolic Ca2%2b and Ca2%2b-activated Cl- current dynamics: Insights from two functionally distinct mouse exocrine cells
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The dynamics of Ca2%2b release and Ca2%2b-activated Cl- currents in two related, but functionally distinct exocrine cells, were studied to gain insight into how the molecular specialization of Ca2%2b signalling machinery are utilized to produce different physiological endpoints: in this case, fluid or exocytotic secretion. Digital imaging and patch-clamp methods were used to monitor the temporal and spatial properties of changes in cytosolic Ca2%2b concentration ([Ca2%2b]c) and Cl- currents following the controlled photolytic release of caged-InsP3 or caged-Ca2%2b In parotid and pancreatic acinar cells, changes in [Ca2%2b]c and activation of a Ca2%2b-activated Cl- current occurred with close temporal coincidence. In parotid, a rapid global Ca2%2b signal was invariably induced, even with low-level photolytic release of threshold amounts of InsP3. In pancreas, threshold stimulation generated an apically delimited [Ca2%2b]c signal, while a stronger stimulus induced a global [Ca2%2b]c signal which exhibited characteristics of a propagating wave. InsP3 was more effective in parotid, where [Ca2%2b]c signals initiated with shorter latency and exhibited a faster time-to-peak than in pancreas. The increased potency of InsP3 in parotid probably results from a four-fold higher number of InsP3 receptors as measured by radiolabelled InsP3 binding and western blot analysis. The Ca2%2b sensitivity of the Cl- channels in parotid and pancreas was determined from the [Ca2%2b] -current relationship measured during a dynamic %27Ca2%2b ramp%27 produced by the continuous, low-level photolysis of caged-Ca2%2b. In addition to a greater number of InsP3 receptors, the Cl- current density of parotid acinar cells was more than four-fold greater than that of pancreatic cells. Whereas activation of the current was tightly coupled to increases in Ca2%2b in both cell types, local Ca2%2b clearance was found to contribute substantially to the deactivation of the current in parotid. These data reveal specializations of common modules of Ca2%2b-release machinery and subsequent effector activation that are specifically suited to the distinct functional roles of these two related cell types.
The dynamics of Ca2%2b release and Ca2%2b-activated Cl- currents in two related, but functionally distinct exocrine cells, were studied to gain insight into how the molecular specialization of Ca2%2b signalling machinery are utilized to produce different physiological endpoints: in this case, fluid or exocytotic secretion. Digital imaging and patch-clamp methods were used to monitor the temporal and spatial properties of changes in cytosolic Ca2%2b concentration ([Ca2%2b]c) and Cl- currents following the controlled photolytic release of caged-InsP3 or caged-Ca2%2b In parotid and pancreatic acinar cells, changes in [Ca2%2b]c and activation of a Ca2%2b-activated Cl- current occurred with close temporal coincidence. In parotid, a rapid global Ca2%2b signal was invariably induced, even with low-level photolytic release of threshold amounts of InsP3. In pancreas, threshold stimulation generated an apically delimited [Ca2%2b]c signal, while a stronger stimulus induced a global [Ca2%2b]c signal which exhibited characteristics of a propagating wave. InsP3 was more effective in parotid, where [Ca2%2b]c signals initiated with shorter latency and exhibited a faster time-to-peak than in pancreas. The increased potency of InsP3 in parotid probably results from a four-fold higher number of InsP3 receptors as measured by radiolabelled InsP3 binding and western blot analysis. The Ca2%2b sensitivity of the Cl- channels in parotid and pancreas was determined from the [Ca2%2b] -current relationship measured during a dynamic 'Ca2%2b ramp' produced by the continuous, low-level photolysis of caged-Ca2%2b. In addition to a greater number of InsP3 receptors, the Cl- current density of parotid acinar cells was more than four-fold greater than that of pancreatic cells. Whereas activation of the current was tightly coupled to increases in Ca2%2b in both cell types, local Ca2%2b clearance was found to contribute substantially to the deactivation of the current in parotid. These data reveal specializations of common modules of Ca2%2b-release machinery and subsequent effector activation that are specifically suited to the distinct functional roles of these two related cell types.
