abstract

• The genome of Alphaviruses can be modified to produce self-replicating RNAs and virus-like particles, which are useful virological tools. In this work, we generated three plasmids for the transfection of mammalian cells: an infectious clone of Chikungunya virus (CHIKV), one that codes for the structural proteins (helper plasmid), and another one that codes nonstructural proteins (replicon plasmid). All of these plasmids contain a reporter gene (mKate2). The reporter gene in the replicon RNA and the infectious clone are synthesized from subgenomic RNA. Co-transfection with the helper and replicon plasmids has biotechnological/biomedical applications because they allow for the delivery of self-replicating RNA for the transient expression of one or more genes to the target cells.

authors

• Colunga-Saucedo M.
• Comas García Mauricio
• Rosales Mendoza Sergio

publication date

• 2022-01-01

published in

• Viruses  Journal

keywords

• alphavirus DNA vectors; Chikungunya virus; helper plasmid; replicon plasmid RNA; animal; Chikungunya virus; gene vector; genetic transfection; genetics; mammal; metabolism; plasmid; replicon; virus replication; Animals; Chikungunya virus; Genetic Vectors; Mammals; Plasmids; Replicon; RNA; Transfection; Virus Replication

Digital Object Identifier (DOI)

• 10.3390/v15010132

PubMed ID

• 36680173

start page

end page

volume

• 15

issue

• 1