abstract

• Struthanthus quercicola, a hemiparasitic plant known as seca palo,is used by Nahuatl traditional healers against diabetes, wounds, and rashes. We aimed to investigate the effects of different S. quercicola extracts, which were selected based on their traditional use in Tamazunchale, San Luis Potosí, on the cell viability and antioxidant activity in HeLa cell cultures. S. quercicola growing on Guazuma ulmifolia and Citrus sp. hosts was collected, and methanolic and ethanolic extracts as well as decoctions, infusions, and microwave-assisted extracts were obtained. The terpenoid, alkaloid, flavonoid, saponin, and tannin contents of each extract were evaluated qualitatively and quantitatively. The effects of different extracts on the viability of cervical adenocarcinoma (HeLa) cells were tested using an MTT assay. The differences in the total flavonoid and phenolic contents and free-radical scavenging activity in relation to the host and the extract were also determined. In assessments of the effects of the extracts on cell viability, eight organic extracts (4 from G. quercicola grown on Host 1 and 4 from G. quercicola grown on Host 2) were shown to decrease cell viability significantly in comparison with the control. However, the extract obtained by percolation (PMeOH) caused a significant increase in cell viability (p < 0.05), especially with the plant grown on Host 1. The microwave aqueous and methanolic extracts of the plants grown on both hosts showed a significant increase in the percentage of apoptosis (p < 005). In conclusion, different extracts of Struthanthus quercicola showed variable effects on cell viability and apoptosis. Isolation of the molecule or molecules with inhibitory and proliferative effects on cells should be conducted to evaluate their possible use as antineoplastic agents. © 2022 Luz Eugenia Alcántara-Quintana et al.

authors

• Alcántara Quintana Luz Eugenia
• Arjona-Ruiz C.
• De Loera Carrera Denisse Atenea
• Gamboa León Miriam Rubí de los Milagros
• Terán Figueroa Yolanda

publication date

• 2022-01-01

published in

• Evidence-based Complementary and Alternative Medicine  Journal

keywords

• alcohol; alkaloid; antineoplastic agent; antioxidant; distilled water; flavonoid; free radical; gallic acid; hydroxymethylglutaryl coenzyme A reductase kinase; methanol; organic solvent; plant extract; quercetin; saponin; sterol; Struthanthus quercicola extract; tannin derivative; terpenoid; unclassified drug; antioxidant activity; apoptosis; Article; cell culture; cell proliferation; cell viability; Citrus; clinical assessment; clinical evaluation; comparative study; controlled study; drug effect; free radical scavenging assay; Guazuma ulmifolia; HeLa cell line; host; human; human cell; in vitro study; medicinal plant; microwave assisted extraction; MTT assay; qualitative analysis; quantitative analysis; Struthanthus quercicola; traditional healer

Digital Object Identifier (DOI)

• 10.1155/2022/9679739

PubMed ID

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volume

• 2022

issue