abstract

• Trypanosoma cruzi parasite - causal Chagas disease agent -affects about 7 million people; no vaccine is available, and current medications have not been entirely effective. Multidisciplinary efforts are necessary for developing clinical vaccine prototypes. Thus, this research study aims to assess the expressed and whole-cell administration protection of the oral vaccine prototype Tc24:Co1 using Schizochytrium sp. microalga. High recombinant protein expression yields (675 mu g/L) of algal culture were obtained. Additionally, Schizochytrium sp.-Tc24:Co1 resulted stable at 4 degrees C for up to six months and at 25 degrees C for three months. After receiving four oral doses of the vaccine, the mice showed a significant humoral immune response and a parasitemia reduction associated with a lack of heart inflammatory damage compared with the unvaccinated controls. The Schizochytrium sp.-Tc24:Co1 vaccine demonstrates to be promising as a prototype for further development showing protective effects against a T. cruzi challenge in a mouse model.

authors

• Angulo C.
• Dumonteil E.
• Monreal-Escalante E.
• Rosales Mendoza Sergio

publication date

• 2024-01-01

published in

• Microbial Pathogenesys  Journal

keywords

• Neglected tropical diseases; Parasites; Vaccine efficacy; Algae-made vaccine; Chagas

Digital Object Identifier (DOI)

• 10.1016/j.micpath.2023.106488

PubMed ID

• 38061668

start page

end page

• 106488

volume

• 186

issue