abstract

• Fluorine (F-) is considered a toxic chemical element, the exposure to this chemical compound in humans goes almost unnoticed; at present, an important number of people are exposed to water sources contaminated with F- causing health damage, and the effects on bone tissue are considered the most significant. Objective: To evaluate the effect of NaF in different osteoinduction processes. Materials and methods: An ATCC hFOB1.19 cell line was used which were cultured with the different NaF concentrations (1, 1.5, 3, 5, 10, 15 mg/l). Quantitative viability through the Alamar-Blue test and qualitative cytotoxicity according to ISO10993-5:2009, intra and extracellular Ca 2 concentrations through the Arsenazo III test, qualitative and quantitative osteogenesis processes in osteoblasts with the Millipore osteogenesis kit, immunocytochemistry to determine the expression of RUNX-2, and Mito-Tracker assay to evaluate the quantity of mitochondria in the treated cells were performed. Results and conclusions: Fluoride significantly altered cellular morphological patterns in sub-chronic exposure, it was also capable of reducing the expression of the RUNX2 protein and the Ca 2 concentration of intracellular and extracellular as well as being able to reduce the number of mitochondria in a dose-dependent way.

authors

• Escobar García Diana María
• Palestino Escobedo Alma Gabriela
• Pozos Guillén Amaury de Jesús

publication date

• 2023-01-01

published in

• Fluoride  Journal

keywords

• Ca 2, Mitochondria, Morphological changes, Osteoinduction, RUNX2

Digital Object Identifier (DOI)

PubMed ID

start page

• 714

end page

• 727

volume

• 56

issue

• 4