Optical imaging of acute epileptic networks in mice
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The potential of intrinsic optical imaging and resting-state analysis under anesthetized conditions as a tool to study brain networks associated with epileptic seizures is investigated. Using an acute model of epileptiform activity, the 4-aminopyridine model in live mice, we observe the changes in resting-state networks with the onset of seizure activity and in conditions of spiking activity. Resting-state networks identified before and after the onset of epileptiform activity show both decreased and increased homologous correlations, with a small dependence on seizure intensity. The observed changes are not uniform across the different hemodynamic measures, suggesting a potential decoupling between blood flow and metabolism in the low-frequency networks. This study supports the need for a more extensive investigation of epileptic networks including more than one independent hemodynamic measurement. © The Authors.
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4-aminopyridine; animal models of epilepsy.; biomedical imaging; functional connectivity; laser speckle contrast; optical intrinsic signals; resting state 4-Aminopyridine; Animal model; Biomedical imaging; Functional connectivity; Intrinsic signal; Laser speckle contrast; Resting state; Medical imaging; Optical image storage; 4 aminopyridine; oxygen; oxyhemoglobin; 4 aminopyridine; acute disease; animal; article; blood; brain circulation; brain cortex; C57BL mouse; chemically induced disorder; epilepsy; fluorescence imaging; male; metabolism; methodology; mouse; pathology; physiology; vascularization; brain cortex; chemically induced; epilepsy; fluorescence imaging; metabolism; pathology; procedures; 4-Aminopyridine; Acute Disease; Animals; Cerebral Cortex; Cerebrovascular Circulation; Epilepsy; Male; Mice; Mice, Inbred C57BL; Optical Imaging; Oxygen; Oxyhemoglobins; 4-Aminopyridine; Acute Disease; Animals; Cerebral Cortex; Cerebrovascular Circulation; Epilepsy; Male; Mice; Mice, Inbred C57BL; Optical Imaging; Oxygen; Oxyhemoglobins
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