abstract

• The AuNPs have been used in biomedicine as therapeutic tools for cancer. However, its role in the context of respiratory physiology has been little studied. This study aimed to determine the impact of AuNPs on respiratory smooth muscle tone, using a model of isolated tracheal rings from female and male rats precontracted with acetylcholine (ACh). AuNPs exerted a contractile effect only in the concentration of 100 ug/ml. This contractile effect was not modified by gender. The possible mediator could be nitric oxide (NO), measured in a physiological solution containing the tracheal rings treated with different concentrations of AuNPs. The results obtained in this study show that the AuNPs are bio-inert in a concentration range of 0.1−10 μg/mL; however, 100 μg/mL could trigger airway hyperresponsiveness. Similar effects were obtained in isolated trachea rings treated with 100 μg/mL HAuCl4. An evaluation of HAuCl4 in physiological buffer at various HEPES concentrations (0–20 mM) showed the formation of AuNPs that could explain the contractile effect on the tracheal smooth muscle. © 2021 The Authors
• The AuNPs have been used in biomedicine as therapeutic tools for cancer. However, its role in the context of respiratory physiology has been little studied. This study aimed to determine the impact of AuNPs on respiratory smooth muscle tone, using a model of isolated tracheal rings from female and male rats precontracted with acetylcholine (ACh). AuNPs exerted a contractile effect only in the concentration of 100 ug/ml. This contractile effect was not modified by gender. The possible mediator %2bcould be nitric oxide (NO), measured in a physiological solution containing the tracheal rings treated with different concentrations of AuNPs. The results obtained in this study show that the AuNPs are bio-inert in a concentration range of 0.1−10 μg/mL; however, 100 μg/mL could trigger airway hyperresponsiveness. Similar effects were obtained in isolated trachea rings treated with 100 μg/mL HAuCl4. An evaluation of HAuCl4 in physiological buffer at various HEPES concentrations (0–20 mM) showed the formation of AuNPs that could explain the contractile effect on the tracheal smooth muscle. © 2021 The Authors

authors

• González Castillo María del Carmen
• Maldonado-Ortega D.A.
• Martínez Castañón Gabriel Alejandro
• Navarro-Tovar G.

publication date

• 2021-01-01

funding provided via

• Consejo Nacional de Ciencia y Tecnología, CONACYT: PN-2017-01-4710  Grant

published in

• Toxicology Reports  Journal

keywords

• AuNPs; Biosafety; Nitric oxide; Toxicity; Trachea acetylcholine; buffer; cholinergic receptor; ethylenediamine; gallic acid; gold nanoparticle; nitric oxide; silver nanoparticle; animal experiment; animal model; animal tissue; Article; biomedicine; biosafety; blood vessel reactivity; bronchus hyperreactivity; controlled study; drug analysis; drug synthesis; female; hydrodynamics; incubation time; male; muscle contraction; muscle tone; nonhuman; particle size; pH; photon correlation spectroscopy; rat; respiratory function; respiratory tract allergy; smooth muscle tone; surface charge; surface plasmon resonance; temperature; trachea muscle; transmission electron microscopy; ultrasound; ultraviolet visible spectroscopy; zeta potential

Digital Object Identifier (DOI)

• 10.1016/j.toxrep.2021.07.002

PubMed ID

start page

• 1412

end page

• 1418

volume

• 8

issue