B regulatory cells associated with changes in biochemical and inflammatory parameters in normal-glycemic individuals, pre-diabetes and T2DM patients
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Aim: To determine the percentages of (CD19 %2b CD24 %2b CD38%2b, CD19 %2b CD24 %2b CD27%2b, CD19 %2b IL-10%2b)-Breg cells, IL-17 single and IL-17%2b/IFN-γ double producers T cells and IFN-γ%2b T cells, in normal-glycemic individuals, prediabetes and T2DM patients, and to analyze the association of Breg cells with metabolic parameters of T2DM. Methods: percentages of Breg cells, IL-17%2b and IL-17 %2b IFN-γ%2b T cells, IFN-γ%2b T cells and IL-10 were determined by flow cytometry. IL-6 levels were evaluated by ELISA assay. Results: increased IL-6 levels, IL-17%2b and IL-17 %2b IFN-γ%2b T cells and a diminution of IL-10 levels and CD19 %2b IL-10%2b cells in T2DM patients were observed. We found that CD19 %2b CD24 %2b CD27%2b cells and CD19 %2b CD24 %2b CD38%2b cells were increased in T2DM patients. The percentages of CD19 %2b CD24 %2b CD38%2b cells were associated with HOMA-B, TyG index, HDL and cholesterol values. In normal-glycemic individuals, CD19 %2b CD24 %2b CD27%2b cells were inversely associated to triglycerides and TyG index. In prediabetes patients, CD19 %2b CD24 %2b CD38%2b cells were inversely related with cholesterol and LDL. Finally, CD19 %2b CD24 %2b CD38%2b cells were inversely related with HDL values in T2DM patients. Conclusion: Our results suggest that increased percentages of IL-17 single and IL-17/IFN-γ double producers T cells in T2DM patients may be a consequence of the initial CD19 %2b IL-10%2b cells reduction. Furthermore, dyslipidemia could play an important role in percentages and activity of B regulatory cells. © 2021 Elsevier B.V.
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Beta-cell functionality; Breg cells; IL-10; Insulin resistance; Th17 cells; Type 2 Diabetes Mellitus ADP ribosyl cyclase/cyclic ADP ribose hydrolase 1; CD19 antigen; CD24 antigen; CD27 antigen; gamma interferon; glucose; high density lipoprotein; interleukin 10; interleukin 6; low density lipoprotein; triacylglycerol; adult; Article; cell function; cholesterol blood level; clinical article; controlled study; cytokine production; dyslipidemia; enzyme linked immunosorbent assay; female; flow cytometry; glucose blood level; homeostasis model assessment; human; human cell; impaired glucose tolerance; lipoprotein blood level; lymphocyte count; lymphocyte function; male; metabolic parameters; non insulin dependent diabetes mellitus; pancreas islet beta cell; regulatory B lymphocyte; T lymphocyte; triacylglycerol blood level; impaired glucose tolerance; inflammation; metabolism; non insulin dependent diabetes mellitus; regulatory B lymphocyte; Adult; B-Lymphocytes, Regulatory; Diabetes Mellitus, Type 2; Female; Humans; Inflammation; Male; Prediabetic State
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