abstract

• Cell mono-layers were irradiated with nanosecond laser pulses under two distinct scenarios: (a) with culturing medium positioning the beam waist at different stand-off distances γ and (b) without cell culturing medium, positioning the beam waist directly on top of the cell mono-layer. Damaged cells were marked with Trypan Blue, a vital cell marker. Three different zones of damage were identified: (1) a zone of complete cell clearance, surrounded by (2) a ring of dead cells marked with Trypan Blue and (3) the rest of the cell culture where the cells remain alive and viable. Different hydrodynamic mechanisms damage cells as it was shown by high speed video for γ=0 and comparison with time resolved imaging. The cell damage mechanism has its origin on the optical breakdown plasma formation. For the case with culturing medium, a combination of plasma formation and shear stresses are responsible for cell damage; wheras for the case without cell culturing medium, the plasma formation is the only mechanism of interaction between laser pulses and cells. The rapidly expanding plasma generates shock waves whose pressure is most likely responsible for the cell detachment observed. © 2009 SPIE.

authors

• Aguilar G.
• Camacho-López S.
• Evans R.
• Guillén G.
• Pérez Gutiérrez Francisco Gerardo

publication date

• 2009-01-01

published in

• Progress in Biomedical Optics and Imaging - Proceedings of SPIE  Proceedings

keywords

• Bubble dynamics; Cell damage extent; Nanosecond laser pulses; Optical breakdown; Shear stresses; Water jet Bubble dynamics; Cell damage extent; Nanosecond laser pulses; Optical breakdown; Water jet; Cell membranes; Fluid dynamics; Jets; Laser pulses; Lasers; Plasmas; Pulsed laser applications; Shear stress; Strength of materials; Cell culture

Digital Object Identifier (DOI)

• 10.1117/12.816562

start page

end page

volume

• 7175