Ex-vivo multi-modal microscopy of healthy skin Conference Paper uri icon


  • The thorough characterization of skin samples is a critical step in investigating dermatological diseases. The combination of depth-sensitive anatomical imaging with molecular imaging has the potential to provide vast information about the skin. In this proof-of-concept work we present high-resolution mosaic images of skin biopsies using Optical Coherence Tomography (OCT) manually co-registered with standard microscopy, bi-dimensional Raman spectral mapping and fluorescence imaging. A human breast skin sample, embedded in paraffin, was imaged with a swept-source OCT system at 1310 nm. Individual OCT volumes were acquired in fully automated fashion in order to obtain a large field-of-view at high resolution (∼10μm). Based on anatomical features, the other three modalities were manually co-registered to the projected OCT volume, using an affine transformation. A drawback is the manual co-registration, which may limit the utility of this method. However, the results indicate that multiple imaging modalities provide complementary information about the sample. This pilot study suggests that multi-modal microscopy may be a valuable tool in the characterization of skin biopsies. © 2014 SPIE.

publication date

  • 2014-01-01