Morphological changes in erythrocytes of people with type 2 diabetes mellitus evaluated with atomic force microscopy: A brief review
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Prevalence of type 2 diabetes mellitus (T2DM) has been increasing worldwide. Cardiovascular diseases are one of the main causes of death among people with T2DM. Morphological changes in erythrocytes have been associated with higher risk of cardiovascular diseases. Atomic force microscopy (AFM) is a new technique that allows non-invasive imaging of cells and the evaluation of changes in mechanical properties. Aim To evaluate by AFM the erythrocytes morphological changes of people with T2DM Methods Search was conducted from in PubMed, ScienceDirect, Scielo, and Lilacs. Erythrocyte, type 2 Diabetes Mellitus and, Microscopy, Atomic Force were the keywords used for the search. Papers included were cross-sectional studies performed in humans. Results Five of seven articles fulfilled the inclusion criteria. Compared with healthy cells, the erythrocytes from individuals affected by T2DM had morphological changes such as a decreased concave depth, diameter, height and a deformation index, while axial ratio, stiffness, adhesive force, aggregation, and rigidity index were increased. The results regarding the erythrocyte roughness were inconclusive. Conclusions The AFM is an excellent instrument to study the altered erythrocytes of subjects affected by T2DM. Morphology changes in erythrocytes could lead to cardiovascular events, which are major complications in people living with this disease © 2017 Elsevier Ltd
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Atomic force microscopy; Cardiovascular diseases; Erythrocytes; Morphology; Type 2 diabetes mellitus Biomechanics; Blood; Cardiology; Diseases; Morphology; Cardio-vascular disease; Cardiovascular event; Cross-sectional study; Deformation indexes; Erythrocytes; Morphological changes; Non-invasive imaging; Type 2 diabetes mellitus; Atomic force microscopy; atomic force microscopy; blood; cardiovascular disease; cytology; erythrocyte; human; non insulin dependent diabetes mellitus; pathology; procedures; prognosis; risk; Cardiovascular Diseases; Diabetes Mellitus, Type 2; Erythrocytes; Humans; Microscopy, Atomic Force; Prognosis; Risk
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