Expression and function of the costimulatory receptor SLAMF1 is altered in lymphocytes from patients with autoimmune thyroiditis
Review
-
- Overview
-
- Research
-
- Identity
-
- Additional Document Info
-
- View All
-
Overview
abstract
-
Context: Signaling lymphocytic activation molecule family 1 (SLAMF1) is a costimulatory receptor expressed by most immune cells. Its role in autoimmune thyroid disease (AITD) is not well known. Objective: To analyze the expression and function of the costimulatory receptor SLAMF1 in lymphocytes of patients with AITD. Design: Cross-sectional, prospective, single-center study. Setting: Department of Endocrinology, Hospital Universitario de la Princesa, Madrid. Patients: Twenty-eight patients with AITD (17 with Graves disease and 11 with Hashimoto thyroiditis) and 21 controls. Intervention: Multiparametric flow cytometry and immunofluorescence techniques to analyze the expression of SLAMF1 in peripheral blood (n = 28) and thyroid tissue (n = 5) mononuclear cells. Assay of inhibition of cellular proliferation to study the function of SLAMF1 in CD4%2bCD25%2b T regulatory (Treg) cells. Main Outcome Measure: Expression levels and the function of SLAMF1 in lymphocytes in AITD patients and controls. Results: Expression of SLAMF1 was significantly increased in peripheral blood CD4%2b, T helper 17, and CD19%2b B cells from AITD patients. Immunofluorescence microscopy detected the presence of SLAMF1%2b lymphocytes in thyroid inflammatory cell infiltrate. Functional studies showed that SLAMF1 engagement in Treg cells increased their suppressive function in healthy controls but not in AITD patients. Conclusions: The altered expression of SLAMF1, as well as its defective function observed in patients with AITD, may have a relevant role in the defective immune-regulatory function observed in this condition. © 2017 by the Endocrine Society.
publication date
published in
Research
keywords
-
CD150 antigen; CD19 antigen; CD4 antigen; interleukin 2 receptor alpha; CD150 antigen; CD19 antigen; CD19 molecule, human; SLAMF1 protein, human; adult; autoimmune thyroiditis; B lymphocyte; blood; cell infiltration; cell proliferation; clinical article; controlled study; cross-sectional study; female; Graves disease; Hashimoto disease; human; human cell; human tissue; inflammatory cell; lymphocyte; male; mononuclear cell; pathogenesis; prospective study; protein expression; protein function; regulatory T lymphocyte; Review; Th17 cell; thyroid gland tissue; blood; Graves disease; Hashimoto disease; metabolism; middle aged; thyroid gland; Adult; Antigens, CD19; B-Lymphocytes; Cross-Sectional Studies; Female; Graves Disease; Hashimoto Disease; Humans; Male; Middle Aged; Prospective Studies; Signaling Lymphocytic Activation Molecule Family Member 1; T-Lymphocytes, Regulatory; Th17 Cells; Thyroid Gland
Identity
Digital Object Identifier (DOI)
PubMed ID
Additional Document Info
start page
end page
volume
issue