abstract

• The aim of this study was to determine the effect of AgNPs on the epigenome of endothelial cells EA.hy926, including the levels of expression of microRNAs (miRNAs) and global DNA methylation patterns. In addition, evaluation of the expression of inflammatory genes and the levels of VCAM-1 protein (miRNA-126 target) was performed. The expression levels of analyzed miRNAs (microRNAs-126, 155 and 146) were reduced significantly and there were not observed changes in inflammatory gene expression. Regarding the levels of protein vascular cell adhesion molecule 1 (VCAM-1), they increase significantly to 0.5 μM AgNPs at 24 h of exposure. As far as DNA methylation is concerned, we found that AgNPs induce a state of global hyper-methylation. In conclusion, it was demonstrated that direct contact between AgNPs and endothelial cells resulted in the dysregulation of highly enriched and vastly functional miRNAs and DNA hypermethylation, that may have multiple effects on endothelium function and integrity. © 2020

authors

• Cortés-García J.D.
• Fernandez-Macias J.C.
• González-Palomo A.K.
• Méndez-Rodríguez K.B.
• Pérez Maldonado Iván Nelinho
• Saldaña-Villanueva K.

publication date

• 2021-01-01

published in

• Environmental Toxicology and Pharmacology  Journal

keywords

• AgNPs; Citotoxicity; DNA methylation; Endothelial cells; microRNAs; Nanomaterials DNA; microRNA; microRNA 126; microRNA 146; microRNA 155; silver nanoparticle; unclassified drug; vascular cell adhesion molecule 1; Article; cell culture; cell viability; controlled study; cytotoxicity; DNA methylation; EA.hy 926 cell line; epigenetics; epigenome; gene expression; gene expression level; gene function; human; human cell; in vitro study; nanotoxicology; priority journal

Digital Object Identifier (DOI)

• 10.1016/j.etap.2020.103543

PubMed ID

• 33166681

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volume

• 81