abstract

• The aim of this work was to evaluate the expression profile of genes involved in signaling, intracellular and extracellular Ca 2 concentration and apoptosis pathways of osteoblasts in contact with a scaffold made of a composite of BCN/MWCNTs. Osteoblasts were cultivated on BCN, MWCNTs and their mixtures. Osteoblast RNA was extracted for sintering cDNA to amplify genes of interest by PCR; intra- and extracellular calcium (Ca2 ) was also quantified. Regarding the genes that participate in the regulation paths (MAPK and NF-KB), it was found that only the expression of NF-KB was affected in all treatments. The expression of VEGFA increased, except in the treatment of high concentration of MWCNTs, where remained unchanged. The expression of genes Apaf-1 and Bcl-2/Bax and TP53 increased as compared to the control (except for TP53 in BC and C1/MWCNTs) indicating that cells are responding to the presence of BCN-MWCNTs composites scaffolds. The results suggest that osteoblast developed a modification in the expression profile of genes that actively participate in cellular processes such as proliferation, vasculogenesis and apoptosis, which may be modulated by the increase of intra- and extracellular Ca2 concentration. © 2020
• The aim of this work was to evaluate the expression profile of genes involved in signaling, intracellular and extracellular Ca%2b2 concentration and apoptosis pathways of osteoblasts in contact with a scaffold made of a composite of BCN/MWCNTs. Osteoblasts were cultivated on BCN, MWCNTs and their mixtures. Osteoblast RNA was extracted for sintering cDNA to amplify genes of interest by PCR; intra- and extracellular calcium (Ca2%2b) was also quantified. Regarding the genes that participate in the regulation paths (MAPK and NF-KB), it was found that only the expression of NF-KB was affected in all treatments. The expression of VEGFA increased, except in the treatment of high concentration of MWCNTs, where remained unchanged. The expression of genes Apaf-1 and Bcl-2/Bax and TP53 increased as compared to the control (except for TP53 in BC and C1/MWCNTs) indicating that cells are responding to the presence of BCN-MWCNTs composites scaffolds. The results suggest that osteoblast developed a modification in the expression profile of genes that actively participate in cellular processes such as proliferation, vasculogenesis and apoptosis, which may be modulated by the increase of intra- and extracellular Ca2%2b concentration. © 2020

authors

• Castorena-Alejandro C.
• Escobar García Diana María
• Flores Reyes Héctor Eduardo
• Gutiérrez-Hernández J.M.
• Pozos Guillén Amaury de Jesús
• Toriz-González G.

publication date

• 2021-01-01

published in

• Materials Science and Engineering C  Journal

keywords

• Apoptosis; Bacterial cellulose nanofibers (BCN), multi-walled carbon nanotubes (MWCNTs), osteoblast; Cell proliferation; Vasculogenesis Cell death; Gene expression; Multiwalled carbon nanotubes (MWCN); Polymerase chain reaction; Apoptosis pathways; Cellular process; Expression profile; Extracellular; Gene expression profiles; Vasculogenesis; Scaffolds (biology); Cells; Concentration; Contacting; Control Systems; Genes; Interest; Profiles; Scaffolds

Digital Object Identifier (DOI)

• 10.1016/j.msec.2020.111531

PubMed ID

• 33255084

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end page

volume

• 118