Role of the chemical modification of titanium dioxide surface on the interaction with silver nanoparticles and the capability to enhance antimicrobial properties of poly(lactic acid) composites
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In this work, the antimicrobial activity of neat and silanized titanium dioxide deposited with silver nanoparticles was evaluated when it was used as filler in a poly(lactic acid) matrix. The silanization and deposition processes were evaluated by scanning transmission electron microscopy and X-ray photoelectron spectroscopy confirming the chemical modification on the titanium dioxide surface by 3-aminopropyltriethoxy silane and the formation of silver nanoparticles. According to the elemental analysis conducted by energy-dispersive X-ray spectroscopy, more silver, 7.4%25 higher, was deposited on the oxide when this was previously silanized and when 30%25 w/w of silver nitrate was used as a precursor. The antimicrobial effect was confirmed for the nanoparticles through the disk diffusion method and for the composites by drop test, against Staphylococcus aureus and Escherichia coli bacteria; the results showed that the inhibition rate increased by 14.2%25 and 39.1%25 for nanoparticles and by 57.6%25 and 38.8%25 for composites against each bacteria, respectively, when deposition was performed on silanized titanium dioxide. Also, better mechanical properties were obtained in the composites filled with silanized oxide; the best results were obtained in the PLA/sTiO2–Ag 20%25 system with an improvement of 45.7%25 in tensile stress and of 38.73%25 for Young’s modulus. Finally, the toxicity of the composites was evaluated by seeding peripheral blood mononuclear cells; results show evidence that composites filled with these nanoparticles are non-toxic since these do not migrate from the polymeric matrix, which helps to enhance the prolonged surface antibacterial effect and to open a broad perspective of the commercial use of these composites. © 2020, Springer-Verlag GmbH Germany, part of Springer Nature.
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Antimicrobial activity; Poly(lactic acid); Polymer composites; Silver nanoparticles; Titanium dioxide
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