Influence of Human Paraoxonase-1 Polymorphism (Q192R) on Serum Levels of Clinical Biomarkers Indicatives of Cardiovascular Diseases Risk in Mexican Women
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Human paraoxonase-1 (PON1) is a hydrolytic enzyme related in the onset, establishment, and progression of inflammatory illnesses, including cardiovascular diseases (CVD). Therefore, the primary goal of this investigation was to determinate the influence of the PON1 gene polymorphism (Q192R) on prognostic biomarkers of CVD in women (n = 350) from San Luis Potosi, Mexico. For everyone enrolled, anthropometric, clinical, and biochemical measurements were acquired. Q192R polymorphism of the PON1 gene was assessed by real-time PCR using TaqMan probes. Also, quantification and/or calculation of suggested predictive CVD biomarkers were completed. The association between PON1(Q192R) polymorphism with clinical predictive CVD biomarkers was assessed using univariate and multivariate logistic regression models. Genotype PON1 (Q192R) frequencies were: 18%25, 47%25 and 35%25 for QQ, QR, and RR, respectively. Besides, the frequency of the variant R allele was 0.58. Furthermore, robust significant associations were found between PON1 (Q192R) polymorphism with serum ADMA [OR (95%25 CI); 3.50 (1.20–5.00), p < 0.01]; FABP4 [2.50 (2.15–3.95), p < 0.01]; and miR-126 [1.50 (1.15–2.00), p < 0.01] levels after adjusting for probable confounders. Data found in this research strongly support the notion that proposes a crucial role of PON1 enzyme (Q192R polymorphism) as a genetic determinant in the CVD risk in Mexican women. © 2020, Springer Science Business Media, LLC, part of Springer Nature.
Human paraoxonase-1 (PON1) is a hydrolytic enzyme related in the onset, establishment, and progression of inflammatory illnesses, including cardiovascular diseases (CVD). Therefore, the primary goal of this investigation was to determinate the influence of the PON1 gene polymorphism (Q192R) on prognostic biomarkers of CVD in women (n = 350) from San Luis Potosi, Mexico. For everyone enrolled, anthropometric, clinical, and biochemical measurements were acquired. Q192R polymorphism of the PON1 gene was assessed by real-time PCR using TaqMan probes. Also, quantification and/or calculation of suggested predictive CVD biomarkers were completed. The association between PON1(Q192R) polymorphism with clinical predictive CVD biomarkers was assessed using univariate and multivariate logistic regression models. Genotype PON1 (Q192R) frequencies were: 18%25, 47%25 and 35%25 for QQ, QR, and RR, respectively. Besides, the frequency of the variant R allele was 0.58. Furthermore, robust significant associations were found between PON1 (Q192R) polymorphism with serum ADMA [OR (95%25 CI); 3.50 (1.20–5.00), p < 0.01]; FABP4 [2.50 (2.15–3.95), p < 0.01]; and miR-126 [1.50 (1.15–2.00), p < 0.01] levels after adjusting for probable confounders. Data found in this research strongly support the notion that proposes a crucial role of PON1 enzyme (Q192R polymorphism) as a genetic determinant in the CVD risk in Mexican women. © 2020, Springer Science%2bBusiness Media, LLC, part of Springer Nature.
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ADMA; Cardiovascular diseases; Epigenetic; FABP4; miRNAs; PON1 polymorphism adiponectin; aryldialkylphosphatase 1; biological marker; chemerin; cholesterol; fatty acid binding protein 4; glucose; high density lipoprotein cholesterol; lipid; low density lipoprotein cholesterol; microRNA; microRNA 126; n(g),n(g) dimethylarginine; triacylglycerol; very low density lipoprotein cholesterol; adult; Article; atherogenic index; biochemical analysis; cardiovascular risk; cross-sectional study; female; gene expression; gene frequency; genetic association; genetic polymorphism; genetic variability; homozygosity; human; lipid fingerprinting; major clinical study; Mexican; prognosis; protein blood level; protein expression; real time polymerase chain reaction
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