Agave salmiana fructans as gut health promoters: Prebiotic activity and inflammatory response in Wistar healthy rats
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Prebiotic effects of Agave salmiana fructans at five different doses were evaluated by the growth of Bifidobacterium, Lactobacillus, and Clostridium strains and SCFA production in the cecum and proximal colon of healthy Wistar rats. Mucosal integrity, bacterial proliferation, and inflammatory response were also examined. Growth of Bifidobacterium and Lactobacillus strains was improved by 12.5%25 doses of fructans in both cecum and proximal colon tissues, and a significant decrease of Clostridium (P < 0.05) was observed. Increases in mucosal thickness, proliferation, and cell adhesion were mainly observed in the cecum. High concentration of butyric acid and total SCFA were contained in the 12.5%25 doses. This study provides direct evidence of the prebiotic effects of Agave salmiana fructans, demonstrating that a diet supplemented with a 12.5%25 dose of fructans promotes major growth of probiotic bacteria and could be used as a potential prebiotic ingredient under the conditions used in this study. Taken together, these results further indicate the significance of Agave salmiana fructans as a prebiotic ingredient in the regulation and prevention of gastrointestinal diseases, as well as for the design of functional foods. © 2019
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Agave salmiana; Fructans; Prebiotic; SCFA Agave salmiana fructans extract; butyric acid; fructan; plant extract; short chain fatty acid; unclassified drug; fructan; prebiotic agent; volatile fatty acid; animal cell; animal experiment; animal tissue; Article; bacterial growth; bacterial strain; Bifidobacterium; cecum; cell adhesion; cell proliferation; Clostridium; colon tissue; controlled study; diet supplementation; DNA isolation; functional food; gastrointestinal disease; health promotion; human; immunofluorescence; inflammation; intestine flora; Lactobacillus; male; nonhuman; prebiotic activity; rat; real time polymerase chain reaction; scanning electron microscopy; Agave; animal; biosynthesis; chemistry; colon; dietary supplement; drug effect; Gram positive bacterium; growth, development and aging; inflammation; intestine mucosa; metabolism; microbiology; Wistar rat; Agave; Animals; Cecum; Colon; Dietary Supplements; Fatty Acids, Volatile; Fructans; Gram-Positive Bacteria; Inflammation; Intestinal Mucosa; Prebiotics; Rats; Rats, Wistar
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