Insights into ergosterol peroxide’s trypanocidal activity
Article
-
- Overview
-
- Research
-
- Identity
-
- Additional Document Info
-
- View All
-
Overview
abstract
-
Trypanosoma cruzi, which causes Chagas disease, is a significant health threat in many countries and affects millions of people. Given the magnitude of this disease, a broader understanding of trypanocidal mechanisms is needed to prevent and treat infection. Natural endoperoxides, such as ergosterol peroxide, have been shown to be toxic to parasites without causing harm to human cells or tissues. Although prior studies have demonstrated the trypanocidal activity of ergosterol peroxide, the cellular and molecular mechanisms remain unknown. The results of this study indicate that a free-radical reaction occurs in T. cruzi following ergosterol peroxide exposure, leading to cell death. Using a combination of biochemical, microscopic and in silico experimental approaches, we have identified, for the first time, the cellular and molecular cytotoxic mechanism of an ergosterol peroxide obtained from Pleurotus ostreatus (Jacq) P. Kumm. f. sp. Florida. © 2019 by the authors. Licensee MDPI, Basel, Switzerland.
publication date
funding provided via
published in
Research
keywords
-
Chagas disease; Docking; Endoperoxide; Ergosterol peroxide; SPR; Trypanosoma cruzi 5alpha,8alpha epidioxy 22e ergosta 6,22 dien 3beta ol; antitrypanosomal agent; ergosterol; unclassified drug; CYP51 protein, Trypanosoma cruzi; cytochrome P450; ergosterol; ergosterol-5,8-peroxide; affect; Article; cell death; comprehension; computer model; controlled study; drug cytotoxicity; drug mechanism; human cell; molecular docking; nonhuman; parasite; Pleurotus ostreatus; threat; trypanocidal activity; cell membrane; chemical structure; chemistry; cytoplasm; drug effect; metabolism; molecular model; Pleurotus; Trypanosoma cruzi; X ray crystallography; Cell Membrane; Crystallography, X-Ray; Cytochrome P-450 Enzyme System; Cytoplasm; Ergosterol; Models, Molecular; Molecular Docking Simulation; Molecular Structure; Pleurotus; Trypanosoma cruzi
Identity
Digital Object Identifier (DOI)
PubMed ID
Additional Document Info
start page
end page
volume
issue