Urinary metabolites altered during the third trimester in pregnancies complicated by gestational diabetes mellitus: Relationship with potential upcoming metabolic disorders
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Gestational diabetes mellitus (GDM) is a disorder in pregnancy with highest impact in the future life of both mother and newborn. Increasing incidence, economic impact, and potential for severe GDM-related pregnancy complications are some factors that have motivated the deep study of physiopathology, risk factors for developing GDM, and potential biomarkers for its diagnosis. In the present pilot study, we analyzed the urinary metabolome profile of GDM patients in the 3rd trimester of pregnancy, when GDM is already established and the patients are under dietary and pharmacological control. An untargeted metabolomics method based on liquid chromatography–mass spectrometry analysis was developed to identify differentially expressed metabolites in the GDM group. We identified 14 metabolites that are significantly upregulated in the urine of GDM patients, and, more importantly, we identified those related with the steroid hormone biosynthesis and tryptophan (TRP) metabolism pathways, which are associated with GDM pathophysiology. Thus, these metabolites could be screened as potential prognostic biomarkers of type two diabetes mellitus, coronary artery disease and chronic renal failure in future follow-up studies with GDM patients. © 2019 by the authors. Licensee MDPI, Basel, Switzerland.
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Gestational diabetes mellitus; Mass spectrometry; Metabolomics; Tryptophan; Urine 11 oxo androsterone glucuronide; 11b, 17a,21 triydroxypreg nonolone; 5 carboxy alpha chromanol; androstenediol; aspartame; benzopyran derivative; biological marker; ceramide; cortodoxone; cortolone 3 glucuronide; creatinine; cucurbitacin; diacylglycerol; glucose; hemoglobin; metformin; sphingomyelin; steroid; tetrahydroaldosterone 3 glucuronide; tryptophan; unclassified drug; urea; urobilinogen; tryptophan; adult; Article; body mass; chronic kidney failure; clinical article; controlled study; coronary artery disease; cross-sectional study; diastolic blood pressure; down regulation; electrospray; female; follow up; gestational age; human; incidence; leukocyte count; metabolic disorder; metabolite; metabolomics; oral glucose tolerance test; pregnancy; pregnancy complication; pregnancy diabetes mellitus; pregnancy disorder; prognosis; protein expression; protein modification; quadrupole mass spectrometry; risk factor; signal transduction; steroidogenesis; systolic blood pressure; third trimester pregnancy; time of flight mass spectrometry; tryptophan metabolism; ultra performance liquid chromatography; upregulation; urinalysis; urinary metabolite; high performance liquid chromatography; mass spectrometry; metabolism; pregnancy diabetes mellitus; procedures; urine; Adult; Chromatography, High Pressure Liquid; Diabetes, Gestational; Female; Humans; Mass Spectrometry; Metabolic Networks and Pathways; Metabolomics; Pregnancy; Pregnancy Trimester, Third; Tryptophan
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