Antimycotic Activity Potentiation of Allium sativum Extract and Silver Nanoparticles against Trichophyton rubrum
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A natural and biocompatible extract of garlic as a support, decorated with silver nanoparticles, is a proposal to generate an effective antifungal agent against dermatophytes at low concentrations. Silver nanoparticles (AgNPs) with a diameter of 26±7 nm were synthesized and their antimycotic activity was examined against Trichophyton rubrum (T. rubrum), inhibiting 94 %25 of growth at a concentration of 0.08 mg ml −1 . Allium sativum (garlic) extract was also obtained (AsExt), and its MIC was 0.04 mg ml −1 . To increase the antifungal capacity of those systems, AsExt was decorated with AgNPs, obtaining AsExt-AgNPs. Using an AsExt concentration of 0.04 mg ml −1 in independent experiments with concentrations from 0.01 to 0.08 mg ml −1 of AgNPs, it was possible to inhibit T. rubrum at all AgNPs concentrations; it proves a synergistic effect between AgNPs and AsExt. Even if 1 %25 of the minimum inhibitory concentration of AsExt (0.0004 mg ml −1 ) is used, it was possible to inhibit T. rubrum at all concentrations of AgNPs, demonstrating the successful antimycotic activity potentiation when combining AsExt and AgNPs. © 2019 Wiley-VHCA AG, Zurich, Switzerland
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Allium sativum (garlic); antimycotic activity; biological activity; potentiation; silver nanoparticles; Trichophyton rubrum garlic extract; silver nanoparticle; antifungal agent; nanoparticle; plant extract; silver; antifungal activity; antimicrobial activity; aqueous solution; Article; biofilm; cell proliferation; cell viability; controlled study; cytotoxicity assay; fungal structures; fungus growth; human; human cell; infrared spectroscopy; minimum inhibitory concentration; peripheral blood mononuclear cell; surface plasmon resonance; synergistic effect; transmission electron microscopy; Trichophyton rubrum; ultraviolet visible spectroscopy; X ray photoemission spectroscopy; chemical structure; chemistry; dose response; drug effect; garlic; isolation and purification; microbial sensitivity test; structure activity relation; Trichophyton; Antifungal Agents; Dose-Response Relationship, Drug; Garlic; Microbial Sensitivity Tests; Molecular Structure; Nanoparticles; Plant Extracts; Silver; Structure-Activity Relationship; Trichophyton
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