The heterelogous expression of the Debaryomyces hansenii DhARO4 gene in nicotiana tabacum improves growth yield, even after inhibition by saline stress [La expresión heteróloga del gen DhARO4 de debaryomyces hansenii en nicotiana tabacum mejora el crecimiento, incluso después de inhibición por estrés salino] Article uri icon

abstract

  • The dumping of dyes into bodies of water represents an environmental and health problem due to its toxicity. A solution to this problem could be the application of mycorremediation. So, in this work Aspergillus niger CDBB-H-175l was investigated in the discoloration (DC) of vat blue dye. A combination of statistical methodologies was applied to optimize DC; initiating with a 24002D It has recently been reported that the expression of the gene codifying for the first enzyme of tyrosine synthesis pathway Aro4p [3-deoxy-D-arabinoheptulosonate-7 phosphate synthase] is involved in the response of the extreme-tolerant yeast Debaryomyces hansenii to salt stress. In addition, and considering that microorganisms and plants share the pathway of synthesis of aromatic amino acids, the DhARO4 gene codifying for the enzyme Aro4p was incorporated into the Nicotiana tabacum plant. It was hypothesized that the resulting plant would better face the salinity derived from irrigation with 100 mM NaCl as compared to pure water irrigation without affecting growth. The genetically modified plants were larger size than the wild type plants under both types of irrigation. © 2019 Revista Mexicana de Ingeniera Quimica. All rights reserved.

publication date

  • 2019-01-01