Calcium sustained release, pH changes and cell viability induced by chitosan-based pastes for apexification Article uri icon

abstract

  • We explored chitosan-based sustained release pastes for apexification. The study aimed to formulate chitosan-based pastes loaded with calcium hydroxide (CH) or with calcium chloride (CC), and to evaluate the sustained release of Ca 2 and pH changes in deionized water as well as the effect of the pastes on cell viability. The pastes were formulated by dissolution of the chitosan in 1%25 or 2%25 acetic acid (AAC) plus the addition of CH or CC, then were suspended in deionized water for 50 days; the released Ca(II) and pH were measured with an electrode probe. The effect of the pastes on viability of human dental pulp cells was evaluated with a MTS assay. The results showed that the pastes prepared with 1%25 and 2%25 AAC and loaded with CH released a 74.9%25 and a 76.1%25 of the Ca 2 content, respectively, while the pastes prepared with 1%25 and 2%25 AAC loaded with CC released a content of Ca 2 of 90.8%25 and 76.6%25, respectively. A control paste (CH and polyethylene glycol) released a 95.4%25; significant statistical differences were found between the percentage of the experimental pastes and the control. The CH-loaded pastes caused an alkaline pH at the starting of the study, but the pH became neutral at the ending. The pH of the CC-loaded pastes was neutral at the starting and was acid at the ending. The pastes no affected on the cell viability. The chitosan-based pastes showed a suitable sustained release profile and cytocompatibility. © 2018, The Society of The Nippon Dental University.
  • We explored chitosan-based sustained release pastes for apexification. The study aimed to formulate chitosan-based pastes loaded with calcium hydroxide (CH) or with calcium chloride (CC), and to evaluate the sustained release of Ca 2%2b and pH changes in deionized water as well as the effect of the pastes on cell viability. The pastes were formulated by dissolution of the chitosan in 1%25 or 2%25 acetic acid (AAC) plus the addition of CH or CC, then were suspended in deionized water for 50 days; the released Ca(II) and pH were measured with an electrode probe. The effect of the pastes on viability of human dental pulp cells was evaluated with a MTS assay. The results showed that the pastes prepared with 1%25 and 2%25 AAC and loaded with CH released a 74.9%25 and a 76.1%25 of the Ca 2%2b content, respectively, while the pastes prepared with 1%25 and 2%25 AAC loaded with CC released a content of Ca 2%2b of 90.8%25 and 76.6%25, respectively. A control paste (CH and polyethylene glycol) released a 95.4%25; significant statistical differences were found between the percentage of the experimental pastes and the control. The CH-loaded pastes caused an alkaline pH at the starting of the study, but the pH became neutral at the ending. The pH of the CC-loaded pastes was neutral at the starting and was acid at the ending. The pastes no affected on the cell viability. The chitosan-based pastes showed a suitable sustained release profile and cytocompatibility. © 2018, The Society of The Nippon Dental University.

publication date

  • 2019-01-01