Altered levels of sirtuin genes (SIRT1, SIRT2, SIRT3 and SIRT6) and their target genes in adipose tissue from individual with obesity
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Introduction: Sirtuins regulate energy metabolism and insulin sensitivity through their ability to act as energy sensors and regulators in several metabolic tissues. Aim: To evaluate the expression levels of sirtuin genes SIRT1, SIRT2, SIRT3 and SIRT6 and their target genes (PPAR-α, PGC1-α, NRF1, DGAT1, PPAR-γ and FOXO3a) in subcutaneous adipose tissue collected from individuals with normoweight, overweight and obesity. Methods: Adipose tissue samples, obtained by lipoaspiration during liposuction surgery, were processed to obtain RNA, which was reverse-transcribed to cDNA. Then, we measured the expression levels of each gene by qPCR. Results: We found differences in the mRNA expression of SIRT1, SIRT2, SIRT3 and SIRT6 and their target genes (PPAR-α, PGC1-α, NRF1, DGAT1, PPAR-γ and FOXO3a) in adipose tissue from overweight or obese subjects when compared to normoweight subjects. All genes analyzed, except SIRT2, showed correlation with BMI. Conclusions: Our findings in human subcutaneous adipose tissue show that increased body mass index modifies the expression of genes encoding sirtuins and their target genes, which are metabolic regulators of adipose tissue. Therefore, these could be used as biomarkers to predict the ability of adipose tissue to gain mass of adipose tissue. © 2018
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Adipose tissue; Lipid metabolism; Lipoaspiration; Obesity; Sirtuins complementary DNA; peroxisome proliferator activated receptor; peroxisome proliferator activated receptor alpha; peroxisome proliferator activated receptor gamma coactivator 1alpha; RNA; sirtuin 1; sirtuin 2; sirtuin 3; sirtuin 6; transcription factor FKHRL1; transcription factor Nrf1; SIRT1 protein, human; SIRT2 protein, human; SIRT3 protein, human; SIRT6 protein, human; sirtuin; sirtuin 1; sirtuin 2; sirtuin 3; adipose tissue; adult; Article; body mass; controlled study; correlation analysis; female; gene expression; human; human tissue; insulin sensitivity; obesity; polymerase chain reaction; priority journal; subcutaneous fat; adipose tissue; biosynthesis; genetics; metabolism; middle aged; obesity; physiology; young adult; Adipose Tissue; Adult; Body Mass Index; Female; Humans; Middle Aged; Obesity; Sirtuin 1; Sirtuin 2; Sirtuin 3; Sirtuins; Young Adult
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