Association of CCR5 G59029A and RANTES –28 C/G polymorphisms in patients with chronic periodontitis and/or type 2 diabetes mellitus, in a Southeastern Mexican population [Asociación de los polimorfismos CCR5 G59029A y rantes –28 C/G en pacientes con periodontitis crónica y/o diabetes mellitus tipo 2 en una población del sureste Mexicano]
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The aim of this study was to determine the association of polymorphisms CCR5G59029A and RANTES –28C/G in chronic periodontitis (CP) and type 2 diabetes mellitus (T2D). A case control study was carried out that included 163 patients with CP and/or T2D. A periodontal examination was done on all potential participants in order to diagnose CP. To confirm the diagnosis of T2D, peripheral blood samples were collected for laboratory analyses (blood glucose and glycosylated hemoglobin). Genotyping was performed by PCR-RFLP. The Genotype AA of CCR5 G59029A increased the risk for CP 7.36 times (95%25 CI = 1.14–78.27, p = 0.03). This genotype’s A allele also increased T2D risk 2.23 times (95%25 CI = 1.10–4.54, p = 0.02). Genotype CG of RANTES –28 C/G increased CP risk 5.99 times (95%25 CI = 1.93–18.57, p = 0.002). Its G allele also raised CP risk 4.55 times (95%25 CI = 1.58–13.04, p = 0.002). In patients suffering both CP and T2D, the CG genotype was associated with the diseases, increasing their risk 4.68-fold (95%25 CI = 1.60–14.03, p = 0.004). Its G allele raised CP and T2D risk 3.8 times (95%25 CI = 1.35–10.67, p = 0.008). The AA genotype and allele A of G59029A polymorphism of CCR5 gene is a risk factor for CP. The CG genotype and allele G polymorphism RANTES –28 C/G gene are risk factors for CP in the population studied. The CG genotype and G allele polymorphism RANTES –28 C/G showed a strong association and a subsequent risk factor for the coexistence of CP and T2D. © 2018, Instituto de Investigaciones Clinicas. All rights reserved.
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Diabetes; Periodontitis; Polymorphism; Rantes chemokine receptor CCR5; chemokine receptor CCR5 gene; genomic DNA; glucose; hemoglobin A1c; RANTES; unclassified drug; adult; aged; anthropometric parameters; Article; body mass; case control study; chemokine receptor CCR5 gene; chronic periodontitis; controlled study; DNA extraction; female; gene; gene frequency; gene mutation; genetic association; genetic polymorphism; genetic risk; genotype; hemoglobin blood level; human; major clinical study; male; non insulin dependent diabetes mellitus; polymerase chain reaction; protein expression; RANTES gene; restriction fragment length polymorphism; risk assessment
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