In vitro evaluation of osteoblastic cells on bacterial cellulose modified with multi-walled carbon nanotubes as scaffold for bone regeneration

In this paper we explore the use of native bacterial cellulose (BC) in combination with functionalized multi-walled carbon nanotubes (MWNTs) as an original biomaterial, suitable three-dimensional (3D) scaffold for osteoblastic cell culture. Functionalized MWNTs were mixed with native BC (secreted by Gluconacetobacter xylinus) with the aim of reinforcing the mechanical properties of BC. The results indicate that BC-MWNTs scaffolds support osteoblast viability, adhesion and proliferation at higher levels as compared to traditional culture substrates. Chemically functionalized MWNTs are also an excellent material to be used as scaffold because these did not affect cell viability and showed an enhanced osteoblast adhesion. These results suggest the potential for this combination of biomaterials, i.e. BC and carbon nanomaterials, as scaffolds for bone regeneration. © 2017 Elsevier B.V.

Bacterial cellulose; Bone regeneration; Cell adhesion; Functionalized MWNTs; Osteoblastic cells Biomaterials; Biomechanics; Bone; Carbon nanotubes; Cell adhesion; Cell culture; Cells; Cellulose; Cytology; Multiwalled carbon nanotubes (MWCN); Nanotubes; Yarn; Bacterial cellulose; Bone regeneration; Carbon nano-materials; Functionalized; Functionalized multi-walled carbon nanotubes; Gluconacetobacter xylinus; Osteoblastic cells; Three-dimensional (3D) scaffolds; Scaffolds (biology); Adhesion; Bacteria; Cells; Cellulose; carbon nanotube; cellulose; bone regeneration; cell line; chemistry; cytology; Gluconacetobacter xylinus; human; materials testing; metabolism; osteoblast; tissue scaffold; ultrastructure; Bone Regeneration; Cell Line; Cellulose; Gluconacetobacter xylinus; Humans; Materials Testing; Nanotubes, Carbon; Osteoblasts; Tissue Scaffolds

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