Cell viability and hemocompatibility evaluation of a starch-based hydrogel loaded with hydroxyapatite or calcium carbonate for maxillofacial bone regeneration Article uri icon

abstract

  • The objective of this study is to evaluate the cell viability and hemocompatibility of starch-based hydrogels for maxillofacial bone regeneration. Seven starch-based hydrogels were prepared: three loaded with 0.5, 1 and 2%25 calcium carbonate (Sigma Aldrich, St. Louis, MO, USA); three loaded with 2, 3 and 4%25 hydroxyapatite (Sigma Aldrich); and one not loaded as a control. A 10 M NaOH was then added to induce hydrogel formation. Human osteoblasts were cultured on each hydrogel for 72 h. An MTS assay (Cell Titer96; PROMEGA, Madison, WI, USA) was used to assess cell viability. Hemocompatibility testing was conducted with normal human blood in the following conditions: 100 mg of each hydrogel in contact with 900 µL of whole blood for 15 min at 37 °C under lateral stirring. Higher percentages of cell viability were observed in starch-based hydrogels loaded with hydroxyapatite as compared with the control. The hemolysis test showed a hemolysis level lower than 2%25. Activated partial thromboplastin time and prothrombin time were unchanged, while platelet counting showed a slight decrease when compared with controls. © 2017, The Society of The Nippon Dental University.

publication date

  • 2017-01-01