Evaluation of vascular tone and cardiac contractility in response to silver nanoparticles, using Langendorff rat heart preparation
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Silver nanoparticles (AgNPs) have been widely used because of their antimicrobial properties. However, several reports suggest that AgNPs exposure promote cardiac effects that involve nitric oxide (NO) and oxidative stress (OS). Nevertheless, there are no studies related to AgNPs-induced effects in cardiac physiology. The aim of this study was to evaluate the AgNPs direct actions on coronary vascular tone and cardiac contractility using Langendorff rat heart preparation. Low concentrations of AgNPs (0.1 and 1 μg/mL) increased NO derived from inducible NO-synthase (iNOS), without modifying cardiac parameters. Meanwhile, high concentrations (10 and 100 μg/mL) promoted a sustained vasoconstriction and increased cardiac contractility related to OS, leading to rhabdomyolysis. Furthermore, AgNPs were internalized in the cardiac muscle, hindering classic actions induced by phenylephrine (Phe) and acetylcholine (ACh). These data suggest that AgNPs affect cardiac physiology in function of the concentration and in part of the NO generation, NOS expression and OS. © 2017 Elsevier Inc.
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Coronary vascular tone; Myocardial contractility; Nitric oxide; Oxidative stress; Silver nanoparticles Metal nanoparticles; Nanoparticles; Nitric oxide; Oxidative stress; Physiology; Rats; Silver; Anti-microbial properties; Cardiac contractility; Cardiac physiology; Low concentrations; Myocardial contractility; Silver nanoparticles; Silver nanoparticles (AgNps); Vascular tones; Heart; acetylcholine; inducible nitric oxide synthase; phenylephrine; silver nanoparticle; inducible nitric oxide synthase; metal nanoparticle; nitric oxide; Nos2 protein, rat; silver; animal tissue; Article; blood vessel tone; cardiac muscle; cardiotoxicity; concentration (parameters); controlled study; heart muscle contractility; heart tissue; isolated heart; male; nonhuman; oxidative stress; rat; rhabdomyolysis; vasoconstriction; animal; chemistry; drug effects; heart; in vitro study; metabolism; muscle contraction; vasoconstriction; Wistar rat; Animals; Heart; In Vitro Techniques; Male; Metal Nanoparticles; Muscle Contraction; Nitric Oxide; Nitric Oxide Synthase Type II; Oxidative Stress; Rats; Rats, Wistar; Silver; Vasoconstriction
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