Evaluation of decellularized matrix and β-tricalcium phosphate as biomaterials for bone neoformation. In vivo study [Evaluación de la Matriz Descelularizada y β-Fosfato Tricálcico como Biomateriales para Neoformación Ósea. Estudio in vivo]

The aim of this study was to evaluate histologically the effect of two biomaterials, a biomaterial derived from porcine Urinary submucosa Bladder Matrix (UBM) and beta-TriCalcium Phosphate (β-TCP), on bone defects. Twenty male New Zealand rabbits were used; the models were divided in two groups: the UBM group; the β-TCP group, and a Negative Control (NC) group. Fivemm defects were created in the femur of each model and then the different biomaterials were set in place depending on each group. At 4 and 8 weeks, the animals in the models were sacrificed and samples of the defect site were collected to perform a Hematoxylin and Eosin stain (H%26E). Histologically, β-TCP group at 4 and 8 weeks presented neoformation of bone-like and cartilage-like tissue, with the presence of inflammatory infiltrate; at 4 and 8 weeks, the UBM group presented neoformation of bone-like and cartilage-like tissue with a low presence of inflammatory infiltrate, and the NC group presented the formation of connective tissue and, in a low proportion, neoformation of bone tissue and cartilage. Both biomaterials, UBM and β-TCP, exhibited the capacity to promote bone neoformation; however, the UBM-based biomaterial produced a better-organized tissue with a lower inflammatory response compared with the β-TCP group. © 2017, Universidad de la Frontera. All rights reserved.

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