Oral Candida isolates and fluconazole susceptibility patterns in older Mexican women Article uri icon

abstract

  • Objectives: To assess the epidemiologic and microbiologic profile and in vitro fluconazole susceptibility of yeasts isolated from the oral mucosa colonization/infection of elderly patients. Background: It has been reported that in older adults increases the oral colonization by Candida particularly C. non-albicans, showing a decreased response to fluconazole, which increases the risk of recalcitrant local and disseminated candidiasis. Materials and methods: This was a prospective cross-sectional study conducted in 120 elderly patients. Oral samples were obtained of mucosal Candida colonization or infection by swabbing. Each sample was plated on CHROMagar® Candida and incubated (36 ± 1.5 °C) for two days. The yeast species were identified using the API®ID32-C-AUX. Fluconazole susceptibility was tested using a broth microdilution assay according to the CLSI methods. Results: The yeast colonization/infection frequency in the total population was 65.8%25. The frequency of the highest Candida carriers was 67.4%25 in the 70-79-year-old-group. Oral candidiasis was present in 20%25, with a tendency to increase with age (33.3%25 of adults aged > 80 years), it was determined that the use of prosthesis is associated with a higher colonization rate (Chi2, p = 0.011). The frequency of colonization/infection cases with more than one species showed a tendency to increase with age, 18.9%25 in the 60-69 year-old-group, 20.9%25 in the 70-79-year-old-group and 29.2%25 in the ≥80 year-old-group. About fluconazole susceptibility: for C. albicans, 20.3%25, about Candida non-albicans species 15.3%25 were dose dependently susceptible (DDS) and 17.9%25 were resistant. Conclusions: After 80 years of age, there is a considerable increase in Candida non-albicans species and a reduced susceptibility to fluconazole. © 2016 Elsevier Ireland Ltd.

publication date

  • 2016-01-01