Chemometrics; Endogenous fluorescence; Graphical user interface; Linear spectral unmixing; Quadratic optimization Fluorescence; Graphical user interfaces; MATLAB; Quadratic programming; Chemometrics; Endogenous fluorescence; Human breast cancer cells; Linear mixture modeling; Linear spectral unmixing; Quadratic approximation; Quadratic optimization; Tissue characterization; User interfaces; algorithm; Article; blind end member and abundance extraction; breast cancer cell line; case study; computer interface; computer program; coronary artery; ex vivo study; fluorescence imaging; fluorescence microscopy; human; human cell; information processing; mathematical analysis; mathematical parameters; mouth mucosa; nonhuman; process optimization; quadratic blind linear unmixing; tissue characterization; web browser; algorithm; biopsy; computer assisted diagnosis; computer graphics; computer simulation; image enhancement; procedures; reproducibility; sensitivity and specificity; statistical model; Algorithms; Biopsy; Computer Graphics; Computer Simulation; Humans; Image Enhancement; Image Interpretation, Computer-Assisted; Linear Models; Microscopy, Fluorescence; Reproducibility of Results; Sensitivity and Specificity; User-Computer Interface