abstract

• Eugenol (mixed with zinc oxide powder) is widely used as direct capping material during pulp therapy in primary teeth. The aim of the present study was to evaluate the effect of eugenol on diverse genes involved in inflammatory and cell apoptosis processes. The regulatory effect of eugenol on the expression of inflammation and apoptotic genes was evaluated in dental pulp fibroblasts from extracted third molars, cultured under concentration of eugenol of 13 μM. Eugenol allowed the expression of inflammatory and apoptotic genes when compared with positive and negative controls. Eugenol is a proinflammatory agent when it is in direct contact with healthy tissues and behaves as an anti-inflammatory agent in tissues undergoing inflammatory/apoptotic processes, as in cases of pulp inflammation in primary teeth. These findings are relevant for dentistry, when considering the application of safer pulp treatments to grossly carious children%27s teeth. © 2016 Andrea Martínez-Herrera et al.
• Eugenol (mixed with zinc oxide powder) is widely used as direct capping material during pulp therapy in primary teeth. The aim of the present study was to evaluate the effect of eugenol on diverse genes involved in inflammatory and cell apoptosis processes. The regulatory effect of eugenol on the expression of inflammation and apoptotic genes was evaluated in dental pulp fibroblasts from extracted third molars, cultured under concentration of eugenol of 13 μM. Eugenol allowed the expression of inflammatory and apoptotic genes when compared with positive and negative controls. Eugenol is a proinflammatory agent when it is in direct contact with healthy tissues and behaves as an anti-inflammatory agent in tissues undergoing inflammatory/apoptotic processes, as in cases of pulp inflammation in primary teeth. These findings are relevant for dentistry, when considering the application of safer pulp treatments to grossly carious children's teeth. © 2016 Andrea Martínez-Herrera et al.

authors

• Escobar-García D.M.
• Garrocho Rangel José Arturo
• Martínez-Herrera A.
• Pozos Guillén Amaury de Jesús
• Ruíz Rodríguez Ma Del Socorro
• Vertiz Hernández Ángel Antonio

publication date

• 2016-01-01

published in

• Mediators of Inflammation  Journal

keywords

• apoptotic protease activating factor 1; cytokine; Escherichia coli lipopolysaccharide; eugenol; glyceraldehyde 3 phosphate dehydrogenase; immunoglobulin enhancer binding protein; interleukin 1beta; protein p53; tumor necrosis factor; vasculotropin A; antiinflammatory agent; complementary DNA; eugenol; immunoglobulin enhancer binding protein; tumor necrosis factor; antiinflammatory activity; apoptosis; Article; controlled study; cytokine production; drug effect; fibroblast; fibroblast culture; gene expression; human; human cell; human tissue; inflammation; tooth pulp; cell culture; cytology; drug effects; fibroblast; inflammation; metabolism; tooth pulp; Anti-Inflammatory Agents; Apoptosis; Cells, Cultured; Dental Pulp; DNA, Complementary; Eugenol; Fibroblasts; Humans; Inflammation; NF-kappa B; Tumor Necrosis Factor-alpha

Digital Object Identifier (DOI)

• 10.1155/2016/9371403

PubMed ID

• 28044068

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volume

• 2016