Vagal nerve stimulation blocks interleukin 6-dependent synaptic hyperexcitability induced by lipopolysaccharide-induced acute stress in the rodent prefrontal cortex
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The ratio between synaptic inhibition and excitation (sI/E) is a critical factor in the pathophysiology of neuropsychiatric disease. We recently described a stress-induced interleukin-6 dependent mechanism leading to a decrease in sI/E in the rodent temporal cortex. The aim of the present study was to determine whether a similar mechanism takes place in the prefrontal cortex, and to elaborate strategies to prevent or attenuate it. We used aseptic inflammation (single acute injections of lipopolysaccharide, LPS, 10mg/kg) as stress model, and patch-clamp recording on a prefrontal cortical slice preparation from wild-type rat and mice, as well as from transgenic mice in which the inhibitor of IL-6 trans-signaling sgp130Fc was produced in a brain-specific fashion (sgp130Fc mice). The anti-inflammatory reflex was activated either by vagal nerve stimulation or peripheral administration of the nicotinic α7 receptor agonist PHA543613. We found that the IL-6-dependent reduction in prefrontal cortex synaptic inhibition was blocked in sgp130Fc mice, or - in wild-type animals - upon application sgp130Fc. Similar results were obtained by activating the anti-inflammatory reflex - a neural circuit regulating peripheral immune response - by stimulation of the vagal nerve or through peripheral administration of the α7 nicotinic receptor agonist PHA543613.Our results indicate that the prefrontal cortex is an important potential target of IL-6 mediated trans-signaling, and suggest a potential new avenue in the treatment of a large class of hyperexcitable neuropsychiatric conditions, including epilepsy, schizophrenic psychoses, anxiety disorders, autism spectrum disorders, and depression. © 2014.
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Anti-inflammatory reflex; GABA; Interleukin 6; Lipopolysaccharide; Vagal nerve stimulation interleukin 6; lipopolysaccharide; n (3 quinuclidinyl)furo[2,3 c]pyridine 5 carboxamide; protein inhibitor; sgp 130fc; unclassified drug; interleukin 6; lipopolysaccharide; acute stress; animal experiment; animal model; animal tissue; Article; controlled study; electrophysiological procedures; GABAergic transmission; immune response; mouse; nerve excitability; neuropharmacology; nonhuman; patch clamp technique; prefrontal cortex; rat; synaptic inhibition; vagus nerve stimulation; animal; disease model; drug effects; inflammation; metabolism; nerve cell inhibition; pathophysiology; physiological stress; physiology; Sprague Dawley rat; synapse; Animals; Disease Models, Animal; Inflammation; Interleukin-6; Lipopolysaccharides; Mice; Neural Inhibition; Prefrontal Cortex; Rats; Rats, Sprague-Dawley; Stress, Physiological; Synapses; Vagus Nerve Stimulation
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