Dynamics of the NK-cell subset redistribution induced by cytomegalovirus infection in preterm infants
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Human cytomegalovirus (HCMV) infection promotes an expansion of NK-cells expressing the CD94/NKG2C receptor. We prospectively monitored the effects of HCMV on the NK-cell receptor (NKG2C, NKG2A, KIR, LILRB1) distribution in preterm infants. As compared to non-infected moderately preterm newborns (. n=. 19, gestational age: 32-37. weeks), very preterm infants (. n=. 5, gestational age: <32. weeks) suffering symptomatic postnatal HCMV infection displayed increased numbers of NKG2C , KIR NK-cells, encompassed by a reduction of NKG2A NK-cells. A similar profile was observed in HCMV-negative newborns (. n=. 4) with asymptomatic infection, during follow-up at ~4 and 10. months of age. Of note, viremia remained detectable in three symptomatic cases at ~10. months despite the persistent expansion of NKG2C NK-cells. Our study provides original insights on the dynamics of the imprint exerted by primary HCMV infection on the NK-cell compartment, revealing that the expansion of NKG2C NK-cells may be insufficient to control viral replication in very preterm infants. © 2015 American Society for Histocompatibility and Immunogenetics.
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Human cytomegalovirus (HCMV) infection promotes an expansion of NK-cells expressing the CD94/NKG2C receptor. We prospectively monitored the effects of HCMV on the NK-cell receptor (NKG2C, NKG2A, KIR, LILRB1) distribution in preterm infants. As compared to non-infected moderately preterm newborns (. n=. 19, gestational age: 32-37. weeks), very preterm infants (. n=. 5, gestational age: <32. weeks) suffering symptomatic postnatal HCMV infection displayed increased numbers of NKG2C%2b, KIR%2b NK-cells, encompassed by a reduction of NKG2A%2b NK-cells. A similar profile was observed in HCMV-negative newborns (. n=. 4) with asymptomatic infection, during follow-up at ~4 and 10. months of age. Of note, viremia remained detectable in three symptomatic cases at ~10. months despite the persistent expansion of NKG2C%2b NK-cells. Our study provides original insights on the dynamics of the imprint exerted by primary HCMV infection on the NK-cell compartment, revealing that the expansion of NKG2C%2b NK-cells may be insufficient to control viral replication in very preterm infants. © 2015 American Society for Histocompatibility and Immunogenetics.
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Cytomegalovirus; Infant; Infection; NK-cells; Preterm CD161 antigen; ganciclovir; natural killer cell receptor; Article; asymptomatic infection; cell compartmentalization; cell expansion; cell function; cell subpopulation; cellular distribution; clinical article; cohort analysis; comparative study; controlled study; cytomegalovirus infection; disease control; flow cytometry; human; Human cytomegalovirus; infant; natural killer cell; newborn disease; prematurity; priority journal; prospective study; real time polymerase chain reaction; viremia; virus load; virus replication; cell differentiation; cell proliferation; complication; Cytomegalovirus; cytomegalovirus infection; follow up; gestational age; immunology; lymphocyte subpopulation; metabolism; natural killer cell; newborn; physiology; Premature Birth; prematurity; Spain; viremia; virology; Cell Differentiation; Cell Proliferation; Cytomegalovirus; Cytomegalovirus Infections; Follow-Up Studies; Gestational Age; Humans; Infant; Infant, Newborn; Infant, Premature; Killer Cells, Natural; Lymphocyte Subsets; Premature Birth; Prospective Studies; Receptors, Natural Killer Cell; Spain; Viremia
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