CD39 expression on Treg and Th17 cells is associated with metabolic factors in patients with type 2 diabetes
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Th17 cells are involved in the pathogenesis of multiple inflammatory diseases such as type two diabetes (T2D). CD39 Treg cells have been implicated as responsible for suppressing Th17 cells. The aim of this study was to evaluate the number and function of CD4 CD25highCD39 Treg and Th17 cells in peripheral blood mononuclear cells (PBMC) from T2D patients and healthy control subjects. The Th17 cells were detected in PBMC under culture with human anti-CD3/CD28 and PMA/ionomycin and the levels of IL-17 were assessed by ELISA and qPCR. The T2D patients with obesity showed significantly lower percentages of CD39 Treg cells. A negative correlation between CD39 Treg cells and weight, and body mass index was detected. In contrast, the low levels of CD4 IL-17 cells in overweight and obese T2D patients showed a positive correlation with glucose and HbA1c. Additionally, we found a subpopulation of Th17 cells that express CD39 and were correlated with glucose and HbA1c. Our findings suggest that the expression of CD39 on Treg cells and also in CD4 IL-17 cells from T2D patients is related to hyperglycemia as well as to overweight and obesity and therefore may participate as a modulator of the effector capacity of Th17 cells. © 2015 American Society for Histocompatibility and Immunogenetics.
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Th17 cells are involved in the pathogenesis of multiple inflammatory diseases such as type two diabetes (T2D). CD39%2b Treg cells have been implicated as responsible for suppressing Th17 cells. The aim of this study was to evaluate the number and function of CD4%2bCD25highCD39%2b Treg and Th17 cells in peripheral blood mononuclear cells (PBMC) from T2D patients and healthy control subjects. The Th17 cells were detected in PBMC under culture with human anti-CD3/CD28 and PMA/ionomycin and the levels of IL-17 were assessed by ELISA and qPCR. The T2D patients with obesity showed significantly lower percentages of CD39%2b Treg cells. A negative correlation between CD39%2b Treg cells and weight, and body mass index was detected. In contrast, the low levels of CD4%2bIL-17%2b cells in overweight and obese T2D patients showed a positive correlation with glucose and HbA1c. Additionally, we found a subpopulation of Th17 cells that express CD39 and were correlated with glucose and HbA1c. Our findings suggest that the expression of CD39 on Treg cells and also in CD4%2bIL-17%2b cells from T2D patients is related to hyperglycemia as well as to overweight and obesity and therefore may participate as a modulator of the effector capacity of Th17 cells. © 2015 American Society for Histocompatibility and Immunogenetics.
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CD39; CD39 Treg cells; Obesity; Th17 cells; Type 2 diabetes carboxyfluorescein diacetate succinimidyl ester; CD3 antibody; CD39 antigen; CD4 antigen; glucose; hemoglobin A1c; interleukin 17; interleukin 2 receptor alpha; ionomycin; transcription factor FOXP3; apyrase; biological marker; CD39 antigen; cytokine; forkhead transcription factor; FOXP3 protein, human; leukocyte antigen; adult; Article; body mass; body weight; cell proliferation; clinical article; controlled study; cytokine release; diet restriction; enzyme linked immunosorbent assay; fasting plasma glucose; female; flow cytometry; glucose blood level; human; human cell; hyperglycemia; inflammatory cell; male; non insulin dependent diabetes mellitus; obesity; peripheral blood mononuclear cell; priority journal; protein expression; quantitative analysis; real time polymerase chain reaction; regulatory T lymphocyte; Th17 cell; aged; case control study; Diabetes Mellitus, Type 2; gene expression; genetics; immunology; immunophenotyping; lymphocyte count; metabolism; middle aged; phenotype; regulatory T lymphocyte; T lymphocyte subpopulation; Th17 cell; Adult; Aged; Antigens, CD; Apyrase; Biomarkers; Case-Control Studies; Cytokines; Diabetes Mellitus, Type 2; Female; Forkhead Transcription Factors; Gene Expression; Humans; Immunophenotyping; Lymphocyte Count; Male; Middle Aged; Phenotype; T-Lymphocyte Subsets; T-Lymphocytes, Regulatory; Th17 Cells
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CD39; CD39+ Treg cells; Obesity; Th17 cells; Type 2 diabetes carboxyfluorescein diacetate succinimidyl ester; CD3 antibody; CD39 antigen; CD4 antigen; glucose; hemoglobin A1c; interleukin 17; interleukin 2 receptor alpha; ionomycin; transcription factor FOXP3; apyrase; biological marker; CD39 antigen; cytokine; forkhead transcription factor; FOXP3 protein, human; leukocyte antigen; adult; Article; body mass; body weight; cell proliferation; clinical article; controlled study; cytokine release; diet restriction; enzyme linked immunosorbent assay; fasting plasma glucose; female; flow cytometry; glucose blood level; human; human cell; hyperglycemia; inflammatory cell; male; non insulin dependent diabetes mellitus; obesity; peripheral blood mononuclear cell; priority journal; protein expression; quantitative analysis; real time polymerase chain reaction; regulatory T lymphocyte; Th17 cell; aged; case control study; Diabetes Mellitus, Type 2; gene expression; genetics; immunology; immunophenotyping; lymphocyte count; metabolism; middle aged; phenotype; regulatory T lymphocyte; T lymphocyte subpopulation; Th17 cell; Adult; Aged; Antigens, CD; Apyrase; Biomarkers; Case-Control Studies; Cytokines; Diabetes Mellitus, Type 2; Female; Forkhead Transcription Factors; Gene Expression; Humans; Immunophenotyping; Lymphocyte Count; Male; Middle Aged; Phenotype; T-Lymphocyte Subsets; T-Lymphocytes, Regulatory; Th17 Cells
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