Modulation of Kv2.1 channels inactivation by curcumin
Article
-
- Overview
-
- Research
-
- Identity
-
- Additional Document Info
-
- View All
-
Overview
abstract
-
Background The aim of the present study was to assess the effects of curcumin on the voltage-dependent Kv2.1 potassium channel. Methods The whole-cell patch-clamp technique was used to explore the regulation of Kv2.1 channels expressed in HEK293 cells by curcumin. Results Curcumin reduced the Kv2.1 currents; the inhibition occurred with a slow time course and was partially reversible. Curcumin did not alter the kinetics and voltage dependence of activation; however, the kinetics of open- and closed-state inactivation was accelerated by curcumin along with a hyperpolarizing shift in the voltage dependence of inactivation. Curcumin inhibition of Kv2.1 current was not use-dependent. Conclusions Overall, our data suggest that curcumin inhibits Kv2.1 channels by modulating the inactivation gating, which would be expected to impact cellular physiology. © 2015 Institute of Pharmacology, Polish Academy of Sciences.
publication date
funding provided via
published in
Research
keywords
-
Curcumin; Inactivation; Kv2.1; Potassium channels curcumin; potassium channel; potassium channel Kv2.1; unclassified drug; curcumin; Shab potassium channel; action potential; Article; cell function; controlled study; drug inhibition; electric conductance; electric potential; half-inactivation voltage; HEK293 cell line; human; human cell; hyperpolarization; kinetics; protein expression; steady state; whole cell patch clamp; antagonists and inhibitors; channel gating; drug effects; membrane potential; Curcumin; HEK293 Cells; Humans; Ion Channel Gating; Kinetics; Membrane Potentials; Shab Potassium Channels
Identity
Digital Object Identifier (DOI)
PubMed ID
Additional Document Info
start page
end page
volume
issue