Cl− transporter block enhances GABAergic spontaneous activity in rat hippocampal CA3 cells
Article
-
- Overview
-
- Research
-
- Identity
-
- Additional Document Info
-
- View All
-
Overview
abstract
-
Chloride-cation Transporter Blockers (CTBs) furose- mide and ammonium were used to test the effects of changes in the internal [Cl−] on the spontaneous and miniature GABAergic post-synaptic currents (PSCs) of CA3 pyramidal cellsof rat hippocampal slices with the whole-cell patch technique. Application of CTBs in the presence of kynurenic acid raised (65 ± 21%25) the frequency of GABAergic spontaneous PSCs leaving unchanged the miniature frequency, indicating that the increase in synaptic activity was caused by interneurone firing. Partial removal of external chloride yielded the same effect, suggesting that Ecl, contributes to the resting potential of interneurones. PSC rise times were prolonged and their mean amplitude was lowered by furosemide as well as the response to exogenous muscimol, confirming that furosemide exerts some GABAA receptor antagonism. © Rapid Communications of Oxford Ltd.
publication date
published in
Research
keywords
-
CA3; Chloride transport; GABA; Hippocampal slice; Interneurones; Patch-clamp; Synaptic activity 4 aminobutyric acid A receptor; 4 aminobutyric acid A receptor stimulating agent; ammonia; bicuculline; chloride channel; furosemide; kynurenic acid; muscimol; penicillin G; tetrodotoxin; animal tissue; article; brain slice; controlled study; electrophysiology; hippocampus; interneuron; newborn; nonhuman; priority journal; pyramidal nerve cell; rat; Ammonium Compounds; Animal; Biological Transport; Chlorides; Electrophysiology; Furosemide; gamma-Aminobutyric Acid; Hippocampus; Neurons; Rats; Rats, Wistar; Support, Non-U.S. Gov't; Synapses
Identity
Digital Object Identifier (DOI)
PubMed ID
Additional Document Info
start page
end page
volume
issue