Regulatory T-cell subsets in response to specific Mycobacterium tuberculosis antigens in vitro distinguish among individuals with different QTF and TST reactivity
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Regulatory T cells (Tregs), a subset of CD4. %2b T cells related with immune regulation, have been associated with active and latent tuberculosis infection (LTBI). Treg frequencies were evaluated by multicolor flow cytometry (FC) in peripheral blood mononuclear cells (PBMCs) stimulated with mycobacterial antigens ESAT-6, CFP-10, and TB7.7 to assess their capacity to distinguish subjects with different reactivity to the QuantiFERON-TB® Gold In-Tube (QFT-IT) test and the tuberculin skin test (TST). Increased frequencies of CD4. %2b. CD25highCD39. %2b cells were found for the [TST. %2b, QTF. %2b] compared with the [TST. %2b, QTF. -] group. Also, higher frequencies were observed for the [TST. %2b, QTF. %2b] compared with the [TST. %2b, QTF. -] and [TST. -, QTF. -] groups in CD4. %2b. CD25highFoxp3. %2b and CD4. %2b. CD25highCD39. %2b. Foxp3. %2b populations. Receiver operating characteristics (ROC curve) analysis confirmed these discriminating results. QFT-IT and TST quantitative values correlated with several Treg population frequencies. © 2015 Elsevier Inc.
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QFT; Regulatory T cells; TST; Tuberculosis CD39 antigen; culture filtrate protein 10; early secretory antigenic target 6; gamma interferon; Mycobacterium antigen; tb7 7 antigen; transcription factor FOXP3; tuberculin; unclassified drug; apyrase; bacterial antigen; CD39 antigen; forkhead transcription factor; FOXP3 protein, human; IL2RA protein, human; interleukin 2 receptor alpha; interleukin 7 receptor alpha; leukocyte antigen; Mycobacterium tuberculosis antigens; adult; Article; CD4+ CD25+ T lymphocyte; cell stimulation; controlled study; female; flow cytometry; human; human cell; immune function test; in vitro study; lung tuberculosis; major clinical study; Mycobacterium tuberculosis; Mycobacterium tuberculosis test kit; peripheral blood mononuclear cell; phenotype; priority journal; quantiferon tb gold in tube test; quantitative analysis; receiver operating characteristic; regulatory T lymphocyte; T lymphocyte subpopulation; tuberculin test; CD4+ T lymphocyte; cohort analysis; immunology; interferon gamma release assay; latent tuberculosis; male; metabolism; middle aged; mononuclear cell; Mycobacterium tuberculosis; regulatory T lymphocyte; T lymphocyte subpopulation; transmission; young adult; Adult; Antigens, Bacterial; Antigens, CD; Apyrase; CD4-Positive T-Lymphocytes; Cohort Studies; Female; Forkhead Transcription Factors; Humans; In Vitro Techniques; Interferon-gamma Release Tests; Interleukin-2 Receptor alpha Subunit; Interleukin-7 Receptor alpha Subunit; Latent Tuberculosis; Leukocytes, Mononuclear; Male; Middle Aged; Mycobacterium tuberculosis; T-Lymphocyte Subsets; T-Lymphocytes, Regulatory; Tuberculin Test; Tuberculosis, Pulmonary; Young Adult
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