Iron Chelate and coconut water in in vitro germination of Rossioglossum grande (Orchidaceae) [Quelato de hierro y agua de coco en la germinación in vitro de Rossioglossum grande (Orchidaceae)]
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Rossioglossum grande (Lindl.) Garay %26 G.C. Kenn is a Mexican native orchid threatened to extinguish according to the NOM-059-SEMARNAT-2010. In vitro asymbiotic germination is a conservation tool wide-ly used for threatened orchids. Generate basic knowledge about in vitro response of R. grande was the object of the work, comparing seed germination in Knudson C medium (KC) versus Dalla Rosa y Laneri medium (DR), modified adding iron chelate and coconut water. We distinguished three different stages of culture: the sowing culture (CM) and two subcultivated culture (C1 and C2). Experimental design was totally aleatory. Anova, Tukey%27s test (p? 0.05) y Chi-square were used to analyze germination variables averages. After 60 days from sowing (CM), seedling was more developed, and in general it had more per-centage of green and vital protocorms using DR medium. After 12 months without subcultivate, we assist at regeneration of protocorms in DR medium in a significant way. In the subcultivation C1 and C2 we test similar responses. We assume that use of iron chelate and coconut water improves the protocorms devel-opment and their regeneration of R. grande in in vitro conditions.
Rossioglossum grande (Lindl.) Garay %26 G.C. Kenn is a Mexican native orchid threatened to extinguish according to the NOM-059-SEMARNAT-2010. In vitro asymbiotic germination is a conservation tool wide-ly used for threatened orchids. Generate basic knowledge about in vitro response of R. grande was the object of the work, comparing seed germination in Knudson C medium (KC) versus Dalla Rosa y Laneri medium (DR), modified adding iron chelate and coconut water. We distinguished three different stages of culture: the sowing culture (CM) and two subcultivated culture (C1 and C2). Experimental design was totally aleatory. Anova, Tukey's test (p? 0.05) y Chi-square were used to analyze germination variables averages. After 60 days from sowing (CM), seedling was more developed, and in general it had more per-centage of green and vital protocorms using DR medium. After 12 months without subcultivate, we assist at regeneration of protocorms in DR medium in a significant way. In the subcultivation C1 and C2 we test similar responses. We assume that use of iron chelate and coconut water improves the protocorms devel-opment and their regeneration of R. grande in in vitro conditions.
